Aging modulates microglia phenotypes in neuroinflammation of MPTP-PD mice

Experimental Gerontology
Kai Yao, Yong-Fei Zhao

Abstract

As the crucial etiological factor, aging-related microglia activation promotes the development of Parkinson's disease (PD). However, the molecular and functional changes of aged-microglia and their contribution to neurodegeneration in PD are only partially understood, which was investigated in our study. Female C57BL/6 mice were randomly divided into four groups, included young-control group, young-MPTP group, aged-control group and aged-MPTP group. Pole test and adhesive removal test were firstly performed. ELISA assay was used to detect the content of interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) in brain tissue. Then we tested the expression of tyrosine hydroxylase (TH), p-nuclear transcription factor (NF-κB), toll-like receptor2 (TLR2), arginase-1 (arg-1), inducible nitric oxide synthase (iNOS) by western blot and immunofluorescence analysis. Our results showed that aging promoted M1 microglia activation and inhibited M2 microglia activation in SN in MPTP-PD model, accompanied by the elevation of proinflammatory cytokine (TNF-α and IL-1β). Consequently, aging significantly aggravated motor dysfunction and dopaminergic neuron loss in SN. Besides, compared with young-MPTP group, the protein expression of TLR2...Continue Reading

Citations

Jul 6, 2019·Veterinary Pathology·James C TarrantEnrico Radaelli
Jul 10, 2019·Journal of Parkinson's Disease·Silvia CerriFabio Blandini
Jul 3, 2019·Inflammopharmacology·Kai Yao, Heng-Bing Zu
Apr 18, 2020·Experimental Biology and Medicine·Genell Tantingco, Myoung-Gwi Ryou
Jan 31, 2020·Frontiers in Aging Neuroscience·Yumei LiuAnmu Xie
Dec 6, 2019·Frontiers in Cellular Neuroscience·Cai-Yun LiuHong-Liang Zhang
Oct 30, 2020·International Journal of Molecular Sciences·Daisuke IkutameMasamichi Shinoda
Jan 27, 2021·CNS Neuroscience & Therapeutics·Farideh IravanpourAbolhassan Ahmadiani
Dec 29, 2020·Frontiers in Immunology·Banghao JianZhengqi Lu
Apr 8, 2021·Journal of Neuroinflammation·Neus Rabaneda-LombarteCarme Solà
Jul 3, 2021·International Journal of Molecular Sciences·Ahmad JallohPaula C Bickford

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