PMID: 6163460Feb 20, 1981Paper

Aging of erythrocytes results in altered red cell surface properties in the rat, but not in the human. Studies by partitioning in two-polymer aqueous phase systems

Biochimica Et Biophysica Acta
H WalterG S Ascher

Abstract

Aqueous solutions of dextran and of poly(ethylene glycol) when mixed give rise to two-phase systems useful in separating cells, on the basis of their surface properties, by partitioning. Depending on whether salts with unequal or equal affinity for the two phases are chosen, phases with or without an electrostatic potential difference between the phases are obtained. At appropriate polymer concentrations the former yield cell partition coefficients (i.e., the quantity of cells in the top phase as a percentage of total cells added) based on charge-associated surface properties while the latter reflect membrane lipid-related parameters. With increasing cell age, rat erythrocytes have diminishing partition coefficients in both charged and uncharged phases. Using the elevated aspartate aminotransferase levels of younger red cells as a marker, we have not found that young mature erythrocytes of human do not have the highest partition coefficient in the red cell population as they do in rat. Experiments with isotopically labeled dog red cells yield results similar to those found with human erythrocytes. Furthermore, density-separated young and old red cells from human give overlapping countercurrent distribution curves. Finally, coun...Continue Reading

References

Jan 21, 1976·Biochimica Et Biophysica Acta·N S CohenD J Hanahan
Oct 20, 1977·Nature·S J LunerB R Ware
Feb 28, 1973·Biochimica Et Biophysica Acta·R ReithermanS H Barondes
Feb 10, 1970·Biochimica Et Biophysica Acta·C C Winterbourn, R D Batt
Jan 4, 1966·Biochimica Et Biophysica Acta·H Walter, F W Selby
Feb 7, 1967·Biochimica Et Biophysica Acta·H WalterR Garza
Nov 17, 1980·Biochemical and Biophysical Research Communications·H WalterG V Seaman
Nov 1, 1962·Experimental Cell Research·P A ALBERTSSON, G D BAIRD

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Citations

Dec 15, 1993·Mechanisms of Ageing and Development·G BartoszP Soudain
Jan 1, 1986·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·J LindenaI Trautschold
Jun 1, 1986·Analytical Biochemistry·H Walter, G Johansson

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