Agitation and aeration effects in suspension mammalian cell cultures.

Cytotechnology
C P MarquisC Harbour

Abstract

Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effects at the reactor level have been included here. A discussion of the difference between suspension and microcarrier cultures, in relation to hydrodynamic effects, is included.

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Citations

Jun 20, 1992·Biotechnology and Bioengineering·Y ShiS H Park
Mar 11, 2008·Molecular Biotechnology·T TharmalingamM Butler
Nov 1, 1993·Journal of Biotechnology·M al-RubeaiM H Goldman
Nov 12, 2014·Biotechnology and Bioengineering·Tharmala Tharmalingam, Chetan T Goudar
Dec 28, 2017·Biotechnology and Bioengineering·Albert J PaulFriedemann Hesse
Jan 1, 1993·AIDS Research and Human Retroviruses·Z L JonakC Debouck

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