Agmatine affects glomerular filtration via a nitric oxide synthase-dependent mechanism

The American Journal of Physiology
D SchwartzR C Blantz

Abstract

Arginine decarboxylase is present in the kidney and metabolizes the amino acid, arginine, to agmatine. Agmatine increases filtration rate in single nephrons (J. J. Lortie, W. F. Novotny, O. W. Peterson, V. Vallon, K. Malvey, M. Mendonca, J. Satriano, P. Insel, S. C. Thomson, and R. C. Blantz. J. Clin. Invest. 97:413-420, 1996). Experiments were conducted to determine whether exogenously administered agmatine exerts these effects via interaction with nitric oxide synthase (NOS) and whether this interaction depends upon alpha 2-adrenergic receptors. Agmatine microperfused (1 microM) into the urinary space of surface glomeruli of the rat increased nephron filtration rate from 33 +/- 4 to 40 +/- 5 nl/min with complete recovery within 10 min. When NG-monomethyl-L-arginine (L-NMMA), a nonselective NOS inhibitor, was systemically infused, agmatine no longer increased single-nephron glomerular filtration rate (SNGFR). BHT-933, an alpha 2-adrenergic agonist, did not increase SNGFR and was unaffected by concurrent L-NMMA. In vitro incubation of freshly harvested glomeruli with agmatine resulted in significant increases in the generation of cGMP, effects similar to carbachol, and blocked by nitro-L-arginine methyl ester (L-NAME) but not y...Continue Reading

Citations

Jun 6, 2000·American Journal of Physiology. Cell Physiology·M J LortieR C Blantz
Dec 12, 2002·American Journal of Physiology. Renal Physiology·Massimo SabbatiniVittorio E Andreucci
Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sanjay K MistrySidney M Morris
May 6, 2021·International Journal of Molecular Sciences·Pablo MaturanaElena Uribe
Mar 8, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Viswanathan PragasamPalaninathan Varalakshmi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.