Agmatine is transported into liver mitochondria by a specific electrophoretic mechanism

The Biochemical Journal
M SalviAntonio Toninello

Abstract

Agmatine, a divalent diamine with two positive charges at physiological pH, is transported into the matrix of liver mitochondria by an energy-dependent mechanism the driving force of which is DeltaPsi (electrical membrane potential). Although this process showed strict electrophoretic behaviour, qualitatively similar to that of polyamines, agmatine is most probably transported by a specific uniporter. Shared transport with polyamines by means of their transporter is excluded, as divalent putrescine and cadaverine are ineffective in inhibiting agmatine uptake. Indeed, the use of the electroneutral transporter of basic amino acids can also be discarded as ornithine, arginine and lysine are completely ineffective at inducing the inhibition of agmatine uptake. The involvement of the monoamine transporter or the existence of a leak pathway are also unlikely. Flux-voltage analysis and the determination of activation enthalpy, which is dependent upon the valence of agmatine, are consistent with the hypothesis that the mitochondrial agmatine transporter is a channel or a single-binding centre-gated pore. The transport of agmatine was non-competitively inhibited by propargylamines, in particular clorgilyne, that are known to be inhibito...Continue Reading

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Citations

Dec 21, 2007·Amino Acids·M P M MarquesA Toninello
Dec 17, 2009·Amino Acids·E AgostinelliA Toninello
Aug 3, 2011·Amino Acids·Valentina BattagliaAntonio Toninello
Mar 27, 2009·American Journal of Physiology. Cell Physiology·Mary Ann ArndtJoseph Satriano
Sep 18, 2013·Amino Acids·Silvia GrancaraAntonio Toninello
Jan 4, 2012·Pharmacology & Therapeutics·Gerhard J Molderings, Britta Haenisch
Feb 23, 2010·Plant Physiology and Biochemistry : PPB·Annalisa TassoniDonatella Serafini-Fracassini
Mar 21, 2007·Biochemical Society Transactions·M A GrilloA Toninello
Mar 21, 2007·Biochemical Society Transactions·M P M Marques, L A E Batista de Carvalho
Aug 4, 2006·Cancer Research·Itzhak NissimMarc Yudkoff

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