AgNOR cut-point to distinguish mild and moderate epithelial dysplasia

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Amit Chattopadhyay, Jay G Ray

Abstract

Distinguishing mild and moderate epithelial dysplasia is difficult and controversial. We demonstrated earlier that silver-stainable nucleolar organizer region (AgNOR) is a simple, inexpensive and quantifiable method which can standardize this distinction reliably. To establish a cut-point mean AgNOR count for epithelial dysplasia validated by bootstrapping. One hundred oral leukoplakia biopsies at Dr R. Ahmed Dental College, Kolkata, India, examined for epithelial dysplasia using standard method (gold standard) were assessed for their mean AgNOR counts. A cut-point was selected employing receiver operating characteristic (ROC) curve. This estimate was stabilized by 10 000 resamples (with replacement) using parametric and non-parametric bootstrap, a Monte Carlo statistical method, corrected for bias to estimate standard errors (SE) of cut-point. Area under the curve (AUC) was 74%; non-parametric method suggested mean AgNOR cut-point = 2.42 AgNORs/nucleus; parametric method suggested cut-point = 2.57. The cut-point 95% confidence intervals (CIs) (bias corrected and accelerated) from parametric method (2.31; 2.66) was lower than non-parametric (2.43; 2.82). Applying a conservative paradigm, taking the lowest of all bootstrap 95% C...Continue Reading

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Sep 25, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Amit ChattopadhyayDaniel J Caplan
Jan 25, 2003·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Jay G RayDaniel J Caplan
Dec 15, 2004·International Journal of Obesity : Journal of the International Association for the Study of Obesity·F A B ColugnatiJ A de Aguiar Carrazedo Taddei

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Citations

Jun 1, 2011·Journal of Applied Oral Science : Revista FOB·João Adolfo Costa HanemannMireile São Geraldo dos Santos Souza
Jan 7, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Patrícia Carlos CaldeiraMaria Auxiliadora Vieira do Carmo

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