Agonist-evoked changes in cytosolic pH and calcium concentration in human platelets: studies in physiological bicarbonate

The Journal of Physiology
S O SageT J Rink

Abstract

1. Cytosolic pH (pHi) and calcium concentration ([Ca2+]i) have been investigated in the presence and absence of physiological HCO3- in human platelets co-loaded with the fluorescent indicators BCECF and Fura-2. Basal pHi and changes evoked by butyrate, thrombin, platelet activating factor (PAF), ADP and phorbol ester were investigated, as were the effects of removing external Na+. 2. In the presence of physiological HCO3- and CO2, basal pHi was 7.02 +/- 0.04 compared with 7.15 +/- 0.05 in the absence of HCO3-. Estimated cytosolic buffering power was reduced from 35.6 +/- 3.0 to 14.5 +/- 0.4 mM/pH unit by the omission of HCO3-. 3. Thrombin evoked an immediate acidification of 0.03 +/- 0.01 pH units in the presence of HCO3- and 0.07 +/- 0.01 pH units in its absence. The acidifications were followed by a slow alkalinization. The final pHi was 0.10 +/- 0.01 units above basal in the presence of HCO3- and 0.08 +/- 0.02 units above basal in the absence of HCO3-. The initial acidification was significantly greater in the absence of HCO3-. The subsequent increase in pHi was similar in the presence and absence of this ion, but the calculated loss of proton equivalents was greater in the presence of HCO3-. 4. Replacement of extracellular ...Continue Reading

Citations

Dec 1, 1996·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R M Harris, R H Waring
Apr 27, 2005·Thrombosis Research·Oscar A Gende
Sep 3, 2013·The International Journal of Biochemistry & Cell Biology·Ludmila A Kasatkina, Tatiana A Borisova
May 1, 1993·Hypertension·D RosskopfW Siffert
Jan 5, 2000·The Journal of Biological Chemistry·R M HarrisP J Hughes
Apr 29, 1998·Journal of Hypertension·P LijnenV Petrov
Oct 23, 1996·Biochimica Et Biophysica Acta·O AharonovitzY Granot
Jan 12, 1996·Biochimica Et Biophysica Acta·O A Gende, H E Cingolani
Jun 5, 1993·Biochimica Et Biophysica Acta·R NieuwlandJ W Akkerman

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