Agonist-induced aggregation of Chinese hamster ovary cells coexpressing the human receptors for fibrinogen (integrin alphaIIbbeta3) and the platelet-activating factor: dissociation between adhesion and aggregation.

Blood
Susana LarruceaR Parrilla

Abstract

This work reports the establishment of a Chinese hamster ovary (CHO) cell line stably coexpressing the human alphaIIbbeta3 integrin and the platelet-activating factor receptor (PAFR). These cells aggregate in response to PAF in a Ca(++), alphaIIbbeta3, and soluble fibrinogen (Fg)-dependent manner that is prevented by PAF antagonists or alphaIIbbeta3 blockade. The aggregating response is accompanied by enhanced binding of fibrinogen and the activation-dependent IgM PAC1. This model has permitted us to identify, for the first time, intracellular signals distinctly associated with either alphaIIbbeta3-mediated adhesion or aggregation. Nonreceptor activation of protein kinase C (PKC) by phorbol ester produced cellular adhesion and spreading onto immobilized Fg, but it was not a sufficient signal to provoke cellular aggregation. Moreover, inhibition of PKC impeded the PAF stimulation of cellular adhesion, whereas the aggregation was not prevented. The PAF-induced cellular aggregation was distinctly associated with signaling events arising from the liganded Fg receptor and the agonist-induced stimulation of a calcium/calmodulin-dependent signaling pathway. Sustained tyrosine phosphorylation of both mitogen-activated protein kinase (M...Continue Reading

References

Oct 15, 1991·Biochemical and Biophysical Research Communications·R D YeC G Cochrane
May 1, 1989·The Journal of Cell Biology·Y N Danilov, R L Juliano
Oct 1, 1994·Current Opinion in Cell Biology·S J ShattilJ S Brugge
Nov 1, 1994·Trends in Biochemical Sciences·E A ClarkJ S Brugge
May 1, 1994·Biochemical Society Transactions·T Pawson
Jul 1, 1994·Circulation Research·M I FurmanP J Goldschmidt-Clermont
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E MüllerM Rola-Pleszczynski
Aug 7, 1997·International Journal of Cancer. Journal International Du Cancer·Y Q ChenK V Honn
Sep 20, 1997·Nature·S OffermannsM I Simon
Jun 17, 1998·The Journal of Biological Chemistry·W QiJ S Bennett
Jul 22, 1998·Frontiers in Bioscience : a Journal and Virtual Library·M Moroi, S M Jung

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Citations

May 6, 2008·Experimental Cell Research·Susana LarruceaRoberto Parrilla
Sep 27, 2006·Journal of Thrombosis and Haemostasis : JTH·D PabónC González-Manchón
Jun 24, 2006·The Journal of Biological Chemistry·Chia-Fen HsiehChi-Hung Lin

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