Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor receptor. Role of cytoplasmic tail phosphorylation in each process.

The Journal of Biological Chemistry
I IshiiT Shimizu

Abstract

Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor (PAF) receptor were characterized in transfected Chinese hamster ovary cells. Exposure of the cells to PAF led to rapid sequestration of the receptors into the intracellular compartment and desensitization of the response to PAF. The sequestration was inhibited by pretreatments that perturbed the clathrin-mediated pathway. Subsequent removal of PAF by washing with receptor antagonists led to rapid recycling of the sequestered receptors to the cell surface accompanied by resensitization to PAF. To evaluate the potential role of phosphorylation in the receptor cytoplasmic tail during these processes, mutant receptors in which the tails were truncated or substituted, so as to lack serine/threonine residues, were created. PAF phosphorylated the wild-type receptor rapidly and strongly, but the mutants did not. The maximal extent of sequestration of each mutant was lower than that of the wild-type, and one of the substituted mutants showed no sequestration. Furthermore, the sequestration-defective mutant showed evidence of desensitization after agonist stimulation but not resensitization after agonist removal. Thus, agonist-induced phosphoryla...Continue Reading

References

May 1, 1991·Trends in Biochemical Sciences·S M Hurtley
Aug 1, 1987·The Journal of Cell Biology·K SandvigB van Deurs
Jan 1, 1986·Annual Review of Biochemistry·D J Hanahan
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B A Kamen, A Capdevila
Nov 1, 1995·The Journal of Cell Biology·E J SmartR G Anderson
Jun 30, 1995·The Journal of Biological Chemistry·S LiM P Lisanti
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ChunH F Lodish
Jul 1, 1994·The Journal of Cell Biology·W J ChangR G Anderson
Dec 7, 1995·Biochimica Et Biophysica Acta·T Izumi, T Shimizu
Jan 12, 1996·Cell·M S RobinsonM Zerial
Jul 19, 1996·The Journal of Biological Chemistry·L M Tolbert, J Lameh
Nov 15, 1996·The Journal of Biological Chemistry·N TrapaidzeL A Devi
Jul 18, 1997·The Journal of Biological Chemistry·D M StafforiniS M Prescott

❮ Previous
Next ❯

Citations

Feb 2, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Akira Sato, Keiichi Ebina
Dec 1, 2004·Journal of Assisted Reproduction and Genetics·William E RoudebushDaniel B Shapiro
Jul 17, 1999·European Journal of Biochemistry·K IwataT Haga
Dec 17, 2009·The Journal of Biological Chemistry·Nobuaki HirotaMotonao Nakamura
Mar 26, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Franziska SchwartzkopffAndreas Ludwig
Mar 10, 2007·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Kazuhiko SonoyamaIchiro Hisatome
Oct 15, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Jane C FenelonMarilyn B Renfree
Aug 11, 2012·Journal of Cell Communication and Signaling·Denis J DupréJana Stankova
Jan 8, 2004·Critical Reviews in Clinical Laboratory Sciences·Diana M StafforiniStephen M Prescott
May 5, 1999·The Journal of Physiology·M Bünemann, M M Hosey
Aug 13, 2003·The European Journal of Neuroscience·Suzumi M TokuokaTakao Shimizu
Jul 6, 2015·European Journal of Pharmacology·Akira SatoKeiichi Ebina
Aug 27, 2005·Biochemical and Biophysical Research Communications·Monique LenoirAxel Périanin
Dec 3, 2014·European Journal of Pharmacology·Akira SatoKeiichi Ebina
Dec 25, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Basil O IbeJ Usha Raj
Jul 19, 2005·British Journal of Pharmacology·Elizabeth S FernandesJoão B Calixto
Jan 12, 2002·The Journal of Biological Chemistry·Noriyasu OhshimaTakao Shimizu
Nov 21, 2019·Journal of Neurochemistry·Merja JoensuuFrédéric A Meunier
Apr 23, 1999·The Journal of Biological Chemistry·C Le GouillJ Stanková
Jun 20, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Akira Sato, Keiichi Ebina
Aug 24, 2005·The Journal of Biological Chemistry·Gopal K MaratheJeffrey B Travers
Oct 24, 1998·The Journal of Biological Chemistry·M J Shapiro, S R Coughlin
Jul 31, 1999·Journal of Neurochemistry·C HsiehK Mackie
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Q ZhuangB D Mazer
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephan L BrownRicardo M Richardson
Nov 13, 2020·Frontiers in Cellular and Infection Microbiology·Elaine I Tuomanen

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.