Agonist-stimulated ligand binding by the platelet integrin alpha IIb beta 3 in a lymphocyte expression system.

The Journal of Biological Chemistry
E LohJ S Bennett

Abstract

The ligand binding activity of the platelet integrin alpha IIb beta 3 is initiated by agonist-generated intraplatelet signals. We studied this process in vitro by expressing recombinant alpha IIb beta 3 in Epstein-Barr virus-immortalized B lymphocytes. We found that phorbol ester stimulation induced the adhesion of lymphocytes expressing alpha IIb beta 3 to immobilized fibrinogen. Moreover, replacement of the transmembrane and cytoplasmic domains of the alpha and beta subunits of alpha IIb beta 3 with those of alpha L beta 2 significantly increased adherence, whereas replacement of only the cytoplasmic domains significantly decreased adherence. This suggests that transmembrane segments are involved in the agonist-induced modulation of alpha IIb beta 3 activity. Similar results were seen when the alpha IIb beta 3 activation-dependent monoclonal antibody PAC-1 was substituted for immobilized fibrinogen. We also found that the adherence of lymphocytes expressing beta 3 with either of the two alpha IIb/alpha L chimeras was similar to that of cells expressing alpha IIb beta 3, whereas the adherence of cells expressing alpha IIb with either of the two beta 3/beta 2 chimeras was substantially decreased, suggesting that the identity of...Continue Reading

References

Nov 1, 1979·The Journal of Clinical Investigation·J S Bennett, G Vilaire
Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·B J Bormann, D M Engelman
Apr 1, 1992·The American Journal of Physiology·D M HaverstickL S Gray
May 3, 1991·Cell·D R PhillipsR M Scarborough
May 1, 1988·The Journal of Clinical Investigation·A B ZimrinM Poncz
Jul 11, 1989·Nucleic Acids Research·R W WilsonA L Beaudet
May 1, 1989·The Journal of Cell Biology·Y N Danilov, R L Juliano
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B GrasbergerH Metzger
Feb 18, 1972·Science·S J Singer, G L Nicolson
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J S BennettD B Cines
Mar 1, 1994·The Journal of Cell Biology·T E O'TooleM H Ginsberg
Jun 15, 1993·Biochemical and Biophysical Research Communications·A M CieutatS Rifat

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Citations

Jan 1, 1997·Cell Motility and the Cytoskeleton·M Y NiuV T Nachmias
Jan 1, 1996·Trends in Cardiovascular Medicine·J S Bennett
Oct 23, 1997·Current Opinion in Cell Biology·W Kolanus, B Seed
Mar 28, 1997·The Journal of Biological Chemistry·J S BennettW F DeGrado
Jun 17, 1998·The Journal of Biological Chemistry·W QiJ S Bennett
Jul 19, 2012·Biology of the Cell·Jenson Lim, Neil A Hotchin
Aug 1, 1997·Archives of Biochemistry and Biophysics·E S OhA Woods

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