Agonistic Autoantibodies to the β2-Adrenergic Receptor Involved in the Pathogenesis of Open-Angle Glaucoma

Frontiers in Immunology
Anselm JünemannMartin Herrmann

Abstract

Glaucoma is a frequent ocular disease that may lead to blindness. Primary open-angle glaucoma (POAG) and ocular hypertension (OHT) are common diseases with increased intraocular pressure (IOP), which are mainly responsible for these disorders. Their pathogenesis is widely unknown. We screened the sera of patients with POAG and OHT for the prevalence of autoantibodies (AAb) against G protein-coupled receptors (GPCRs) in comparison to controls. Employing frequency modulation of spontaneously contracting neonatal rat cardiomyocytes in vitro, agonistic GPCR AAb were to be detected in roughly 75% of the patients with POAG and OHT, however, not in controls. Using inhibitory peptides the AAb' target was identified as β2 adrenergic receptor (β2AR). The AAb interact with the second extracellular loop of β2AR. The peptides 181-187 and 186-192 were identified as binding sites of the AAb within the extracellular loop II. The binding of the AAb to β2ARs was verified by surface-plasmon-resonance analysis. The isotype of the AAb was (immunoglobulin) IgG3. In an additional pilot principal-of-proof study, including four patients with POAG, the removal of the AAb against the β2AR and other immunoglobulins G by immunoadsorption resulted in a tran...Continue Reading

References

Nov 1, 1978·Archives of Ophthalmology·R L Coakes, R F Brubaker
Aug 1, 1978·Experimental Eye Research·M E YablonskiB Becker
Jan 1, 1992·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·C A McCannelR F Brubaker
Jan 1, 1988·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·J B JonasG O Naumann
Nov 1, 1987·The Journal of Experimental Medicine·M BrüggemannM S Neuberger
May 1, 1995·Australian and New Zealand Journal of Ophthalmology·H A Quigley
Mar 1, 1995·Archives of Ophthalmology·L I LarssonR F Brubaker
May 1, 1996·The British Journal of Ophthalmology·H A Quigley
Aug 1, 1997·Archives of Ophthalmology·L A KerriganM E Pease
Nov 5, 1997·Journal of Glaucoma·H J Varela, M R Hernandez
Nov 21, 1997·Archives of Ophthalmology·L WaymanR Brubaker
Jan 13, 1998·Vision Research·N J SucherE B Dreyer
Jul 3, 1998·European Journal of Pharmacology·D LebesgueJ Hoebeke
Feb 20, 1999·Survey of Ophthalmology·J B JonasS Panda-Jonas
Apr 9, 1999·The Journal of Clinical Investigation·G WallukatF C Luft
Sep 14, 1999·The American Journal of Physiology·L K PutneyM E O'Donnell
Apr 6, 2000·The American Journal of Cardiology·G WallukatR Hetzer
Feb 22, 2001·Science·J C VenterX Zhu
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Jun 8, 2001·Journal of Neuroscience Research·L Yuan, A H Neufeld
Jul 9, 2002·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Julie Y Crider, Najam A Sharif
Oct 9, 2002·Archives of Ophthalmology·Anders HeijlUNKNOWN Early Manifest Glaucoma Trial Group
Jan 14, 2003·Archives of Ophthalmology·M Cristina LeskeUNKNOWN Early Manifest Glaucoma Trial Group
Aug 19, 2003·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Wolfgang RönspeckRudolf Kunze
Jan 1, 1958·Acta Ophthalmologica·L A ERICSON
Jan 27, 2004·Ophthalmology Clinics of North America·Mary K Wirtz, John R Samples
Jan 1, 1951·Documenta Ophthalmologica. Advances in Ophthalmology·H GOLDMANN
Feb 27, 2004·Investigative Ophthalmology & Visual Science·Gabriele Fuchsjäger-MayrlLeopold Schmetterer
Apr 27, 2004·Experimental Eye Research·Mortimer M Civan, Anthony D C Macknight
Apr 19, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Stephanie C JoachimFranz H Grus
Jun 9, 2005·Neurochemistry International·Liliána Z FehérAndrás Palotás

❮ Previous
Next ❯

Citations

Sep 25, 2019·Journal of the American Heart Association·Amanda J Miller, Taylor A Doherty
Jun 3, 2020·Antibodies·Aliya K StanovaYehuda Shoenfeld
Nov 27, 2020·Klinische Monatsblätter für Augenheilkunde·Bettina Hohberger
Jan 7, 2021·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·C Erb, K Konieczka
Mar 18, 2021·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·Anselm G M JünemannR Rejdak
Apr 22, 2021·Journal of Translational Autoimmunity·Gerd WallukatJohannes Müller
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Theresa SchmidtRené Csuk

❮ Previous
Next ❯

Methods Mentioned

BETA
surface-plasmon-resonance
Surface plasmon resonance
enzyme-linked immunosorbent assay
affinity purification

Clinical Trials Mentioned

NCT00494923

Software Mentioned

Globaffin
Octopus

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.