Agreement between chromogenic in situ hybridisation (CISH) and FISH in the determination of HER2 status in breast cancer

British Journal of Cancer
Laurent ArnouldJérôme Couturier

Abstract

Determination of the HER2/neu (HER2) status in breast carcinoma has become necessary for the selection of breast cancer patients for trastuzumab therapy. Amplification of the gene analysed by fluorescence in situ hybridisation (FISH) or overexpression of the protein determined by immunohistochemistry (IHC) are the two major methods to establish this status. A strong correlation has been previously demonstrated between these two methods. However, FISH is not always feasible in routine practice and weakly positive IHC tumours (2+) do not always correspond to a gene amplification. Our study was performed in order to evaluate the contribution of chromogenic in situ hybridisation (CISH), which enables detection of the gene copies through an immunoperoxidase reaction. CISH was performed in 79 breast carcinomas for which the HER2 status was previously determined by IHC and FISH. The results of IHC, FISH and CISH were compared for each tumour. CISH procedures were successful in 95% of our cases. Whatever the IHC results, we found a very good concordance (96%) between CISH and FISH. Our study confirms that CISH may be an alternative to FISH for the determination of the gene amplification status in 2+ tumours. Our results allow us to thi...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·O P KallioniemiJ W Gray
Aug 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P C Roche, J N Ingle
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·T W JacobsS J Schnitt
Feb 5, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·R E JimenezD W Visscher
Jul 11, 2000·Archives of Pathology & Laboratory Medicine·P L FitzgibbonsS J Schnitt
Aug 24, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·R L RidolfiJ M Arber
Oct 31, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G PaulettiD J Slamon
Dec 6, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·J CouturierX Sastre-Garau
Feb 24, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A LebeauU Löhrs
May 16, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R R TubbsT M Grogan
Aug 28, 2001·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A Thor
Oct 2, 2001·Cancer Control : Journal of the Moffitt Cancer Center·N M Diaz
Dec 18, 2001·The Journal of Pathology·J M BartlettT G Cooke
Feb 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Charles L VogelMichael Press
Jun 6, 2002·Journal of the National Cancer Institute·Soonmyung PaikNorman Wolmark
Jun 6, 2002·Journal of the National Cancer Institute·Patrick C RocheEdith A Perez
Jun 18, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Jianxin ZhaoZuorong Shi

❮ Previous
Next ❯

Citations

Dec 29, 2010·Pathology Research International·Sejal Shah, Beiyun Chen
Dec 13, 2006·Journal of Clinical Pathology·Sara Alexandra Vinhas RicardoFernando Carlos Lander Schmitt
Mar 22, 2014·International Journal of Nanomedicine·Sarwat B RizviAlexander M Seifalian
Mar 31, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mitch DowsettMarc J van de Vijver
Jun 10, 2005·Pathology International·Natasa Todorović-RakovićDragica Nikolić-Vukosavljević
Aug 28, 2004·Histopathology·F LewisA M Hanby
Nov 17, 2005·Pathology International·Hisaki IgarashiHaruhiko Sugimura
Jul 27, 2006·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·M DreilichM Bergqvist
Apr 6, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F E RosaM A C Domingues
Apr 30, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Yun GongNour Sneige
Sep 19, 2009·American Journal of Clinical Pathology·Frédérique Penault-LlorcaMarc van de Vijver
Sep 26, 2006·Advances in Anatomic Pathology·Trishe Y-M Leong, Anthony S-Y Leong
Feb 1, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maryou B K LambrosJorge S Reis-Filho
Oct 4, 2005·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·John M S Bartlett
Apr 19, 2015·Breast Cancer Research : BCR·Paolo NuciforoMaurizio Scaltriti
Sep 21, 2006·International Journal of Technology Assessment in Health Care·Magali MorelleMarie-Odile Carrère
Dec 26, 2006·Expert Review of Molecular Diagnostics·Jennifer LaudadioDaynna J Wolff
Apr 17, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Mahdieh SalimiKeivan Majidzadeh-A
Apr 11, 2013·Expert Review of Molecular Diagnostics·Niels T FogedJan T Jørgensen
Sep 30, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Pedro B AleixoAntonio A Hartmann
Jan 13, 2015·Expert Review of Molecular Diagnostics·Francesca Sanguedolce, Pantaleo Bufo
Jun 1, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Anthony S Y LeongMegan Clarke
Dec 23, 2004·Journal of Molecular Histology·Cornelia Hauser-Kronberger, Nadia Dandachi

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.