Agreement between PDL1 immunohistochemistry assays and polymerase chain reaction in non-small cell lung cancer: CLOVER comparison study.

Scientific Reports
I TsimafeyeuS Tjulandin

Abstract

The goal of the CLOVER study was to perform a pairwise comparison of four tests based on the same patient population with non-small cell lung cancer (NSCLC): three validated PDL1 immunohistochemistry (IHC) assays (Ventana SP142, Ventana SP263, Dako 22C3) and one PCR test. Four hundred seventy-three NSCLC samples were obtained from a biobank and were stained using PDL1 IHC assays. Four trained pathologists independently evaluated the percentage of tumor cells (TC) and immune cells (IC) that stained positive at any intensity. PDL1 transcripts were quantified in 437 patients by a standard Taqman RT-PCR assay using SDHA as a reference gene. A concordance analysis was performed to assess (1) the correlation of TC and IC between different assays and (2) the predictive properties of one test for another. "High" RNA expression was detected in 187 of 437 (43%) patients. The percentage of PDL1-positive cells (≥1%) was higher among the IC than the TC in all IHC three assays. The Pearson correlation coefficients (PCC) for TC were 0.71, 0.87, and 0.75 between 22C3/SP142, 22C3/SP263, and SP263/SP142, respectively. The PCC for IC were 0.45, 0.61, and 0.68 for the same pairs. A low correlation was observed between the PCR test and each of the ...Continue Reading

References

Jun 5, 2016·Molecular Diagnosis & Therapy·Evgeny N ImyanitovSergey A Tjulandin
Jan 12, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marianne J RatcliffeJill Walker
Nov 28, 2017·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Shona HendryStephen B Fox
May 17, 2018·Oncology Reports·Kazutoshi IsobeSakae Homma
Sep 14, 2018·Virchows Archiv : an International Journal of Pathology·Larisa ZavalishinaSergei Tjulandin
Jun 4, 2019·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Deepa Rangachari, Daniel B Costa
Jun 4, 2019·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Edward B GaronRina Hui

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Citations

Feb 16, 2021·Chemical Communications : Chem Comm·Parisa MoazzamJ Justin Gooding
Oct 27, 2020·Frontiers in Oncology·Evgeny N ImyanitovIlya V Tsimafeyeu
May 12, 2021·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Joe YeongDaniel S W Tan
Aug 15, 2021·Clinical Genitourinary Cancer·Maria SorokinaJames J Hsieh

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Methods Mentioned

BETA
PCR
reverse transcription PCR

Software Mentioned

CLOVER

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