Agreement of CK5/6, p40, and p63 immunoreactivity in non-small cell lung cancer

Pathology
Katharina KriegsmannMark Kriegsmann

Abstract

Histological subtyping of non-small cell lung cancer (NSCLC) is of utmost importance for therapy stratification. Common immunohistochemical markers to identify squamous lineage are CK5/6, p40, and p63. Although p40 is considered the gold standard by current guidelines, the agreement of all three markers is an important aspect for tumours more difficult to classify. A total of 1244 NSCLC including 569 squamous cell carcinomas (SqCC) and 675 adenocarcinomas were assembled on a tissue microarray and stained with CK5/6, p40, p63, TTF-1, and Napsin-A. Sensitivity and specificity for squamous lineage markers as well as agreement of CK5/6, p40 and p63 were calculated. Sensitivity of CK5/6, p40, and p63 for SqCC was 93%, 94%, and 94% and specificity was 98%, 97%, and 84%, respectively. Positivity for two of these markers was found in at least in 90% of SqCC. Highest agreement was observed for p40 and p63 (Cohen's kappa 0.80). We report a similar sensitivity of CK5/6, p40, and p63, but a decreased specificity of p63 as compared to CK5/6 and p40 for the identification of squamous lineage. Our results support the use of either CK5/6 or p40 over p63 in the routine diagnostic setting.

Citations

Dec 28, 2019·The Journal of International Medical Research·Rui GuoJun Yang
Nov 21, 2019·International Journal of Molecular Sciences·Artem SmirnovEleonora Candi
Jun 18, 2020·Cancer Management and Research·Jialin QiKuansong Wang
Feb 6, 2020·Molecular Medicine Reports·Xiang WangZhijie Xu
Nov 13, 2020·The Oncologist·Erin L SchenkPaul A Bunn
Jul 15, 2021·Pathology Oncology Research : POR·Sören WeidemannSarah Minner

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