Agreement of the Kato-Katz test established by the WHO with samples fixed with sodium acetate analyzed at 6 months to diagnose intestinal geohelminthes

Acta Tropica
Julián Alfredo Fernández-NiñoRubén Darío Heredia

Abstract

The aim of this study was to evaluate the performance of the Kato-Katz test (WHO version) with stool samples from a rural area, fixed with sodium acetate (SAF). The Kato-Katz test was used to compare unfixed samples (conventional test) with the same samples containing SAF fixative at time 0 and at 6 months. The study included stools from 154 subjects. A marginally statistically significant decrease in prevalence was estimated only for hookworm, when comparing unfixed samples versus the SAF fixed samples read at 6 months (p=0.06). A significant reduction in parasite load was found for hookworm (p<0.01) and Trichuris trichiura (p<0.01) between the unfixed and the fixed sample read at 6 months, but not for Ascaris lumbricoides (p=0.10). This research suggests that the SAF fixative solution is a good option for transporting samples for diagnosis, especially in rural areas in developing countries.

References

Jan 1, 1967·Transactions of the Royal Society of Tropical Medicine and Hygiene·V Zamen, C H Cheong
May 22, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J UtzingerH Marti
Apr 21, 2012·Transactions of the Royal Society of Tropical Medicine and Hygiene·Jorge D MachicadoEduardo Gotuzzo

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Citations

Nov 10, 2015·Current Tropical Medicine Reports·Poppy H L Lamberton, Peter M Jourdan
Oct 3, 2017·Biomédica : revista del Instituto Nacional de Salud·Julián A Fernández-NiñoPatricia Reyes

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