Agrobacterium -Mediated Transformation of the Oryza sativa Thaumatin-Like Protein to Canola (R Line Hyola308) for Enhancing Resistance to Sclerotinia sclerotiorum

Iranian Journal of Biotechnology
Rustam AghazadehMehdi Moradyar

Abstract

Background: Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play a key role in plant defense system. Objectives: Transformation of the rice tlp into canola via Agrobacterium-mediated transformation and evaluation of the antifungal activity of the expressed TLP in the transgenic events on the S. sclerotiorum growth was subject to investigation. Materials and methods: The canola (R line Hyola308) was used for transformation experiment. The vector, pBITLPRA1, was used for the stable transformation. The PCR and southern blotting techniques were used to confirm transgene's presence in the transgenic canola events. Antifungal activity of transgenic plants was evaluated by the radial diffusion and spore germination assays. T2 transgenic plants were evaluated by the intact leaf inoculation method in greenhouse assay. Results: In this study, pBITLPRA1 construct containing tlp gene was introduced into canola and the transformed plants were verified by PCR. ...Continue Reading

References

Dec 3, 1999·Applied and Environmental Microbiology·K ThevissenW F Broekaert
Dec 17, 2008·Journal of Hazardous Materials·P C Abhilash, Nandita Singh
Sep 26, 2013·Allergology International : Official Journal of the Japanese Society of Allergology·Hassan G Ashok KumarYeldur P Venkatesh
Mar 1, 1985·Plant Molecular Biology·S O Rogers, A J Bendich
Jul 20, 2016·World Journal of Microbiology & Biotechnology·Rustam AghazadehZahra Moghadassi Jahromi

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Citations

Mar 6, 2021·Journal of Agricultural and Food Chemistry·Li-Na DingXiao-Li Tan
Aug 15, 2021·International Journal of Biological Macromolecules·Robert NawrotAnna Goździcka-Józefiak

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