Agrobacterium-mediated transformation of dormant lateral buds in poplar trees reveals developmental patterns in secondary stem tissues

Functional Plant Biology : FPB
Antanas V SpokeviciusGerd Bossinger

Abstract

In an attempt to devise a method for the rapid creation of somatic transgenic wood sectors of sufficient size that would allow us to detect and analyse altered wood characteristics within them, we have explored the manual wounding and subsequent infection with Agrobacterium of dormant lateral buds in poplar. Following treatment and transformation with a 35S-GUS construct, frequent stable transformation was found in the form of distinct and specific GUS staining patterns in the outer cortex, cambial region (including primary and secondary xylem and phloem) and pith. Sector frequency and size were consistent with anatomical features of dormant lateral buds at the time of manual wounding and Agrobacterium-infection. The suitability of somatic sector analysis for functional genomic studies as well as for studies investigating pattern formation and the developmental fate of various cell-types within poplar stems is discussed.

References

Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·I AllonaR W Whetten
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·J RalphA M Boudet
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·F SterkyJ Lundeberg
Nov 14, 2000·Current Opinion in Plant Biology·K Roberts, M C McCann
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·M HertzbergG Sandberg
Apr 27, 2002·Physiologia Plantarum·Nigel ChaffeyBjörn Sundberg
Nov 27, 2002·Annals of Botany·Gail Taylor
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Eugene J. Szymkowiak, Ian M. Sussex
Aug 24, 2004·Plant Cell Reports·Antanas V SpokeviciusGerd Bossinger
Jan 1, 1971·Annual Review of Genetics·M N Nesbitt, S M Gartler
Dec 13, 2006·Plant Biotechnology Journal·Malcolm M CampbellSteve H Strauss

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Citations

Mar 1, 2011·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·V Girijashankar
Jun 23, 2018·Journal of Experimental Botany·Gerd Bossinger, Antanas V Spokevicius
Jul 4, 2007·The Plant Journal : for Cell and Molecular Biology·Antanas V SpokeviciusGerd Bossinger
Sep 22, 2019·International Journal of Molecular Sciences·Libert B TonfackEshchar Mizrachi

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