Agrobacterium-mediated transformation of élite indica and japonica rice cultivars

Molecular Biotechnology
J ZhangD F Chen

Abstract

A rapid, efficient, routine system has been established for Agrobacterium tumefaciens-mediated production of hundreds of fertile transgenic plants from commercially important rice cultivars, including an indica cultivar, Pusa Basmati 1. Calli induced from embryos of mature rice seeds were cocultivated with A. tumefaciens strain LBA4404 carrying the plasmid pTOK233, then exposed to hygromycin selection followed by an efficient regeneration system. Based on the total number of calli co-cultivated, the transformation frequencies of independent transgenic rice plants including cultivars Pusa Basmati 1, E-yi 105, E-wan 5 and Zhong-shu-wan-geng, were 13.5, 13.0, 9.1, and 9.3%, respectively. T1 seeds were harvested within 7-8 mo of initiation of mature embryo cultures. Data from Southern hybridization analysis proved that foreign genes on T-DNA were stably integrated into the rice genome at low copy/site numbers. Mendelian inheritance of the transgenes was confirmed in T1 progeny.

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Citations

Jan 25, 2006·Plant Cell Reports·Xiaojia GeShiping Wang
Dec 19, 2006·Science in China. Series C, Life Sciences·Caiyan ChenLihuang Zhu
Mar 29, 2005·Nature Biotechnology·Jacqueline A PaineRachel Drake
Apr 12, 2005·Plant Physiology·Patrick SchaubPeter Beyer
Apr 5, 2007·Nature Protocols·Asuka NishimuraMakoto Matsuoka
Oct 1, 2020·Plant Direct·Iny Elizebeth MathewPinky Agarwal
Feb 14, 2015·Molecular Plant Pathology·Hongli JiTina Kyndt

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