PMID: 9440512Jan 24, 1998Paper

aguA, the gene encoding an extracellular alpha-glucuronidase from Aspergillus tubingensis, is specifically induced on xylose and not on glucuronic acid

Journal of Bacteriology
R P de VriesJ Visser

Abstract

An extracellular alpha-glucuronidase was purified and characterized from a commercial Aspergillus preparation and from culture filtrate of Aspergillus tubingensis. The enzyme has a molecular mass of 107 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 112 kDa as determined by mass spectrometry, has a determined pI just below 5.2, and is stable at pH 6.0 for prolonged times. The pH optimum for the enzyme is between 4.5 and 6.0, and the temperature optimum is 70 degrees C. The alpha-glucuronidase is active mainly on small substituted xylo-oligomers but is also able to release a small amount of 4-O-methylglucuronic acid from birchwood xylan. The enzyme acts synergistically with endoxylanases and beta-xylosidase in the hydrolysis of xylan. The enzyme is N glycosylated and contains 14 putative N-glycosylation sites. The gene encoding this alpha-glucuronidase (aguA) was cloned from A. tubingensis. It consists of an open reading frame of 2,523 bp and contains no introns. The gene codes for a protein of 841 amino acids, containing a eukaryotic signal sequence of 20 amino acids. The mature protein has a predicted molecular mass of 91,790 Da and a calculated pI of 5.13. Multiple copies of the gene were i...Continue Reading

References

Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
May 1, 1994·Molecular Microbiology·L H de GraaffJ Visser
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Citations

Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
Feb 22, 2012·Applied and Environmental Microbiology·Astrid R Mach-AignerLeo H de Graaff
Nov 22, 2012·The FEBS Journal·Maija TenkanenPeter Biely
Mar 4, 2010·Proteomics·Thomas SchneiderKathrin Riedel
Mar 29, 2011·Applied Microbiology and Biotechnology·Sun-Li ChongRonald P de Vries
Aug 20, 2005·Preparative Biochemistry & Biotechnology·Małgorzata MierzwaJanusz Szczodrak
Nov 19, 2008·Applied Biochemistry and Biotechnology·Charles C LeeDominic W S Wong
Sep 27, 2015·The FEBS Journal·Xiang ShengFahmi Himo
Feb 1, 2020·Scientific Reports·Martin TegelaarHan A B Wösten
Aug 26, 2020·Journal of Industrial Microbiology & Biotechnology·Heidi ØstbyAnikó Várnai
Apr 7, 2009·FEBS Letters·Olena RyabovaPeter Biely
Jul 6, 2014·Frontiers in Microbiology·Anthi KarnaouriPaul Christakopoulos
Jun 15, 1999·Journal of Bacteriology·S ShulamiY Shoham
Oct 6, 1998·Applied and Environmental Microbiology·N N van PeijL H de Graaff
Dec 3, 1999·Applied and Environmental Microbiology·R P de Vries, J Visser
Mar 22, 2000·Journal of Biotechnology·M Tenkanen, M Siika-aho
Jun 19, 2021·Fungal Biology·M C Tamayo-OrdóñezY J Tamayo-Ordóñez

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