AHAS herbicide resistance endowing mutations: effect on AHAS functionality and plant growth.

Journal of Experimental Botany
Qin YuStephen B Powles

Abstract

Twenty-two amino acid substitutions at seven conserved amino acid residues in the acetohydroxyacid synthase (AHAS) gene have been identified to date that confer target-site resistance to AHAS-inhibiting herbicides in biotypes of field-evolved resistant weed species. However, the effect of resistance mutations on AHAS functionality and plant growth has been investigated for only a very few mutations. This research investigates the effect of various AHAS resistance mutations in Lolium rigidum on AHAS functionality and plant growth. The enzyme kinetics of AHAS from five purified L. rigidum populations, each homozygous for the resistance mutations Pro-197-Ala, Pro-197-Arg, Pro-197-Gln, Pro-197-Ser or Trp-574-Leu, were characterized and the pleiotropic effect of three mutations on plant growth was assessed via relative growth rate analysis. All these resistance mutations endowed a herbicide-resistant AHAS and most resulted in higher extractable AHAS activity, with no-to-minor changes in AHAS kinetics. The Pro-197-Arg mutation slightly (but significantly) increased the K(m) for pyruvate and remarkably increased sensitivity to feedback inhibition by branched chain amino acids. Whereas the Pro-197-Ser and Trp-574-Leu mutations exhibite...Continue Reading

References

May 15, 1988·Analytical Biochemistry·B K SinghD L Shaner
Jun 7, 1992·American Journal of Health Promotion : AJHP·L A Tucker, M Bagwell
Feb 20, 2002·FEBS Letters·Yu Ting Lee, Ronald G Duggleby
Jul 20, 2002·Annals of Botany·William A Hoffmann, Hendrik Poorter
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jennifer A McCourtRonald G Duggleby
Apr 28, 2007·Psychiatric Services : a Journal of the American Psychiatric Association·Daniel Cukor
Feb 1, 2008·Plant Physiology and Biochemistry : PPB·Ronald G DugglebyLuke W Guddat
Oct 15, 2009·The New Phytologist·Martin M Vila-AiubStephen B Powles
Mar 3, 2010·Annual Review of Plant Biology·Stephen B Powles, Qin Yu
Jul 1, 1999·The American Naturalist·Colin B Purrington, Joy Bergelson

❮ Previous
Next ❯

Citations

Oct 4, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Carlos A SalaBrigitte Weston
May 21, 2013·Plant Science : an International Journal of Experimental Plant Biology·Gabriela BrecciaGraciela Nestares
Apr 12, 2014·Pest Management Science·Paul NeveMartin M Vila-Aiub
Dec 18, 2013·Pest Management Science·Qin Yu, Stephen B Powles
Oct 11, 2015·Pesticide Biochemistry and Physiology·Songhong WeiHaining Wang
May 20, 2015·Biochimica Et Biophysica Acta·Irshad Ahmed BaigMoon-Young Yoon
Jun 15, 2015·Pesticide Biochemistry and Physiology·Wenwen XiaLiyao Dong
Jun 9, 2014·Plant Science : an International Journal of Experimental Plant Biology·Ana C OchogavíaGraciela Nestares
Jul 9, 2013·Trends in Genetics : TIG·Christophe DélyeValérie Le Corre
Jul 30, 2016·Evolution; International Journal of Organic Evolution·Megan L Van EttenRegina S Baucom
Sep 15, 2017·Plant, Cell & Environment·Heping HanStephen B Powles
Dec 14, 2017·Frontiers in Plant Science·Silvia PanozzoMaurizio Sattin
Jan 10, 2018·Pest Management Science·Roman B VercellinoAlejandro Presotto
Sep 7, 2018·Pest Management Science·Gabriela BrecciaGraciela Nestares
Feb 10, 2019·The New Phytologist·Martin M Vila-AiubStephen B Powles
Aug 1, 2019·Plant Biotechnology Journal·Rukmini MishraKaijun Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Software Mentioned

SAS
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.