AHCC Activation and Selection of Human Lymphocytes via Genotypic and Phenotypic Changes to an Adherent Cell Type: A Possible Novel Mechanism of T Cell Activation

Evidence-based Complementary and Alternative Medicine : ECAM
Loretta OlamigokeAlamelu Sundaresan

Abstract

Active Hexose Correlated Compound (AHCC) is a fermented mushroom extract and immune supplement that has been used to treat a wide range of health conditions. It helps in augmentation of the natural immune response and affects immune cell activation and outcomes. The goal of this project was to study and understand the role and mechanisms of AHCC supplementation in the prevention of immunosuppression through T cell activation. The method described here involves "in vitro" culturing of lymphocytes, exposing them to different concentrations of AHCC (0 μg/mL, 50 μg/mL, 100 μg/mL, 250 μg/mL, and 500 μg/mL) at 0 hours. Interestingly, clumping and aggregation of the cells were seen between 24 and 72 hours of incubation. The cells lay down extracellular matrix, which become adherent, and phenotypical changes from small rounded lymphocytes to large macrophage-like, spindle shaped, elongated, fibroblast-like cells even beyond 360 hours were observed. These are probably translated from genotypic changes in the cells since the cells propagate for at least 3 to 6 generations (present observations). RNA isolated was subjected to gene array analysis. We hypothesize that cell adhesion is an activation and survival pathway in lymphocytes and th...Continue Reading

References

Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A L PrietoK L Crossin
Sep 15, 1993·Journal of Immunological Methods·Y ShiE A Turley
Oct 4, 2002·Nutrition·Andrea T BorchersM Eric Gershwin
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Mar 26, 2013·Nature Reviews. Immunology·Rebecca J Brownlie, Rose Zamoyska

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Methods Mentioned

BETA
in vitro transcription
gene array

Software Mentioned

GenomeStudio
Ingenuity Pathway Analysis (

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