AHR2 required for normal behavioral responses and proper development of the skeletal and reproductive systems in zebrafish

PloS One
Gloria R GarciaRobert L Tanguay

Abstract

The aryl hydrocarbon receptor (AHR) is a conserved ligand-activated transcription factor required for proper vertebrate development and homeostasis. The inappropriate activation of AHR by ubiquitous pollutants can lead to adverse effects on wildlife and human health. The zebrafish is a powerful model system that provides a vertebrate data stream that anchors hypothesis at the genetic and cellular levels to observations at the morphological and behavioral level, in a high-throughput format. In order to investigate the endogenous functions of AHR, we generated an AHR2 (homolog of human AHR)-null zebrafish line (ahr2osu1) using the clustered, regulatory interspaced, short palindromic repeats (CRISPR)-Cas9 precision genome editing method. In zebrafish, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) mediated toxicity requires AHR2. The AHR2-null line was resistant to TCDD-induced toxicity, indicating the line can be used to investigate the biological and toxicological functions of AHR2. The AHR2-null zebrafish exhibited decreased survival and fecundity compared to the wild type line. At 36 weeks, histological evaluations of the AHR2-null ovaries revealed a reduction of mature follicles when compared to wild type ovaries, suggesting AHR2...Continue Reading

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Citations

Mar 26, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jaclyn P Souder, Daniel A Gorelick
Aug 22, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·M KarlgrenP Artursson
Oct 27, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katharina DachPamela J Lein
Jul 17, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Danielle HagstromEva-Maria S Collins
Feb 3, 2019·International Journal of Molecular Sciences·Wataru Yoshioka, Chiharu Tohyama
Sep 26, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Prarthana ShankarRobyn L Tanguay
Dec 20, 2019·Environmental Science & Technology·Libe Aranguren-AbadíaOdd André Karlsen
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Jul 14, 2020·Current Opinion in Toxicology·Tamara TalPamela J Lein
Dec 7, 2018·Environmental Science & Technology·Courtney RoperRobert L Tanguay

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Methods Mentioned

BETA
PCR

Software Mentioned

ggplot2
ViewPoint ZebraBox
ViewPoint
R
multcomp
BZ
x Analyzer
sandwich
ImageJ

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