AID and RAG1 do not contribute to lymphomagenesis in Emu c-myc transgenic mice

Oncogene
Rajeev M NepalA Martin

Abstract

DNA breaks caused by recombination-activating gene 1 (RAG1) and activation-induced cytidine deaminase (AID) induce c-myc/immunoglobulin (Ig) heavy chain chromosomal translocations and thereby stimulate lymphomagenesis. However, constitutive expression of c-myc alone is not sufficient to induce lymphomas. Because RAG1 and AID activity occurs outside of Ig genes, we assessed whether these enzymes provide the secondary genetic lesions in Emu c-myc transgenic mice to promote lymphoma development. We found that the tumor incidence and tumor phenotype in Emu c-myc transgenic mice is similar in AID+/+, AID+/- and AID-/- backgrounds in both specific pathogen-free and conventional animal facilities, indicating that AID does not contribute to lymphoma development in Emu c-myc transgenic mice. To examine the role of RAG proteins in Emu c-myc mice, we examined Emu c-myc transgenic mice that harbor the Ig-HEL heavy- and light-chain transgenes, and thus have reduced RAG expression in B cells. We found that tumor incidence was not affected by these Ig transgenes. However, we found that RAG1-/- Emu c-myc mice exhibited accelerated tumor development compared to controls. This data combined with our finding that Emu c-myc mice lived longer in th...Continue Reading

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Citations

Jul 25, 2009·Nature Reviews. Immunology·David H Raulet, Nadia Guerra
Oct 20, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rajeev M NepalAlberto Martin
Apr 21, 2012·Current Opinion in Hematology·Xiwen GuMatthew P Strout
Mar 13, 2014·The Journal of Clinical Investigation·Yuanzhi LuXianghong Zou
Sep 5, 2013·Molecular and Cellular Biology·Staci L PetersRene Opavsky
Oct 12, 2010·The American Journal of Pathology·Jonathan U PeledMatthew D Scharff
Mar 1, 2012·Experimental Hematology·Hongbing LiStuart A Berger
Aug 15, 2014·Frontiers in Immunology·Valentin S Bruttel, Jörg Wischhusen
Jul 26, 2018·Frontiers of Medicine·Sumedha Roy, Yuan Zhuang
Nov 15, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicholas K PreissEdward J Usherwood

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