AID TB resistance line probe assay for rapid detection of resistant Mycobacterium tuberculosis in clinical samples

The Journal of Infection
B Molina-MoyaJ Dominguez

Abstract

To determine the sensitivity and specificity of AID TB Resistance line probe assay (AID Diagnostika, Germany) to detect Mycobacterium tuberculosis and its resistance to first- and second-line drugs in clinical samples using BACTEC 460TB as the reference standard. The test consists on three strips to detect resistance to isoniazid/rifampicin, fluoroquinolones/ethambutol, and kanamycin/amikacin/capreomycin/streptomycin, respectively. This test was performed on 65 retrospectively selected clinical samples corresponding to 32 patients. A valid result was obtained for 92.3% (60/65), 90.8% (59/65) and 78.5% (51/65) of the samples tested, considering the three strips, respectively. Global concordance rates between AID and BACTEC for detecting resistance to isoniazid, rifampicin, fluoroquinolones, ethambutol, kanamycin/capreomycin and streptomycin were 98.3% (59/60), 100% (60/60), 91.5% (54/59), 72.9% (43/59), 100% (51/51) and 98.0% (50/51), respectively. Regarding the discordant results obtained between AID and BACTEC, the alternative molecular methods performed (GenoType MTBDRplus, GenoType MTBDRsl [Hain Lifescience, Germany] and/or pyrosequencing) confirmed the genotypic result in 90.9% (20/22) of the cases. AID line probe assay is ...Continue Reading

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Feb 18, 2016·Transactions of the Royal Society of Tropical Medicine and Hygiene·Derek J Sloan, Joseph M Lewis
Aug 11, 2015·Diagnostic Microbiology and Infectious Disease·A LacomaJ Domínguez
May 6, 2015·The Journal of Infection·B Molina-MoyaJ Dominguez
Apr 2, 2016·Therapeutic Advances in Infectious Disease·Robyn S Lee, Marcel A Behr
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Related Concepts

Drug Resistance
Ethambutol
Isoniazid
Mycobacterium tuberculosis
Rifampin
Streptomycin
Tuberculosis
Tuberculosis, Drug-Resistant
Capreomycin
Isoniazid / Rifampin

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