AIM/LIR-based fluorescent sensors-new tools to monitor mAtg8 functions

Autophagy
Katarzyna Zientara-Rytter, Suresh Subramani

Abstract

Macroautophagy/autophagy, a catabolic process by which cytoplasmic materials are degraded and recycled in lysosomes/vacuoles, remains a rapidly expanding research topic with the need for constantly improved methodologies to study each step of this pathway. Recently Lee and colleagues, as well as Stolz et al., independently reported the development of new AIM/LIR-based fluorescent sensors, which mark individual endogenous mammalian Atg8-family (mAtg8) proteins without affecting the autophagic flux. When expressed in cells, each sensor selectively recognizes individual mAtg8 isoforms and distinguishes mammalian MAP1LC3/LC3 proteins from the related GABARAPs. Such selectivity was achieved by using various LC3-interacting regions with high binding affinity to either a subgroup, or a specific, mAtg8 isoform as part of the sensor. Here we discuss the utility of these sensors in autophagy research and highlight their strengths, weaknesses and future directions.

References

Dec 2, 2000·Nature·Y IchimuraY Ohsumi
Dec 14, 2002·Developmental Cell·Takahiro ShintaniDaniel J Klionsky
Jun 1, 2004·Journal of Cell Science·Yukiko KabeyaTamotsu Yoshimori
Dec 17, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tarek A LeilRichard W Olsen
Jun 21, 2007·The Journal of Biological Chemistry·Serhiy PankivTerje Johansen
Aug 30, 2007·Science's STKE : Signal Transduction Knowledge Environment·Hideki SumimotoTakashi Ito
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Mark A Lemmon
May 30, 2008·Molecular Biology of the Cell·Zhiping XieDaniel J Klionsky
Jun 6, 2008·The Journal of Biological Chemistry·Yoshinobu IchimuraMasaaki Komatsu
Nov 22, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Nobuo N NodaFuyuhiko Inagaki
Jan 20, 2010·FEBS Letters·Nobuo N NodaFuyuhiko Inagaki
Apr 20, 2010·Biochemical and Biophysical Research Communications·Houbo JiangJian Feng
Jun 29, 2010·Autophagy·Hilla WeidbergZvulun Elazar
Aug 18, 2010·The Journal of Cell Biology·Salvatore J CherraCharleen T Chu
Oct 13, 2010·Molecular and Cellular Biology·Faraz K MardakhehJohn K Heath
Sep 12, 2012·Autophagy·Daniel J KlionskyBrian Zuckerbraun
Dec 15, 2012·Journal of Cell Science·Elizabeth DeosaranPeter K Kim
Mar 5, 2014·Autophagy·Ioanna KalvariVasilis J Promponas
Jun 17, 2016·Molecular & Cellular Oncology·Heide Marika Genau, Christian Behrends
Aug 4, 2016·Autophagy·Anne-Claire JacominIoannis P Nezis
Nov 8, 2016·Molecular Cell·Takeshi KaizukaNoboru Mizushima
Nov 24, 2016·ELife·Dorotea FracchiollaSascha Martens

❮ Previous
Next ❯

Citations

Nov 14, 2019·Scientific Reports·Sang-Won ParkDeok-Jin Jang
May 27, 2020·Journal of Physiology and Biochemistry·Md Abdul Alim Al-Bari

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
NMR

Related Concepts

Related Feeds

ATG proteins

The discovery of autophagy-related ('ATG') proteins in the 1990s greatly advanced the mechanistic understanding of autophagy and clarified the fact that autophagy serves important roles in various biological processes.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Related Papers

Journal of Perinatology : Official Journal of the California Perinatal Association
A M Heuchan, R Goldman
Schizophrenia Research
Alison R YungStephen Wood
Physical Review Letters
G LeeS Suto
Physical Review Letters
S J LeeW Greiner
© 2022 Meta ULC. All rights reserved