Air-drying kinetics affect yeast membrane organization and survival

Applied Microbiology and Biotechnology
Guillaume LemetaisPatrick Gervais

Abstract

The plasma membrane (PM) is a key structure for the survival of cells during dehydration. In this study, we focused on the concomitant changes in survival and in the lateral organization of the PM in yeast strains during desiccation, a natural or technological environmental perturbation that involves transition from a liquid to a solid medium. To evaluate the role of the PM in survival during air-drying, a wild-type yeast strain and an osmotically fragile mutant (erg6Δ) were used. The lateral organization of the PM (microdomain distribution) was observed using a fluorescent marker related to a specific green fluorescent protein-labeled membrane protein (Sur7-GFP) after progressive or rapid desiccation. We also evaluated yeast behavior during a model dehydration experiment performed in liquid medium (osmotic stress). For both strains, we observed similar behavior after osmotic and desiccation stresses. In particular, the same lethal magnitude of dehydration and the same lethal kinetic effect were found for both dehydration methods. Thus, yeast survival after progressive air-drying was related to PM reorganization, suggesting the positive contribution of passive lateral rearrangements of the membrane components. This study also s...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·J H CroweL M Crowe
Dec 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·J Piette
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Jun 20, 2000·FEMS Microbiology Ecology·N Gunde-CimermanaA Plemenitasd
Sep 11, 2002·Environmental Microbiology·Helena Santos, Milton S da Costa
Dec 19, 2002·Biochimica Et Biophysica Acta·Yannick MillePatrick Gervais
Oct 17, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A Tunnacliffe, J Lapinski
Nov 25, 2003·Cell Stress & Chaperones·Elenilda de Jesus PereiraElis Cristina Araujo Eleutherio
May 26, 2004·Biochimica Et Biophysica Acta·Jeannine Milhaud
Jul 19, 2005·Nature Biotechnology·Edda KlippStefan Hohmann
Feb 28, 2006·Molecular Ecology·Christian R LandryDuccio Cavalieri
Mar 3, 2006·Biochimica Et Biophysica Acta·R S HerdeiroE C A Eleutherio
Apr 4, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M B FrançaE C A Eleutherio
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J H CroweL M Crowe
Oct 31, 2006·Applied and Environmental Microbiology·Sonia Rodríguez-VargasFrancisca Randez-Gil
Feb 15, 2007·FEMS Yeast Research·Martina TurkNina Gunde-Cimerman
Jun 15, 2007·Environmental Microbiology·Anna A Gorbushina
Jul 8, 2008·Biochimica Et Biophysica Acta·Vishard RagoonananAlptekin Aksan
Jul 16, 2008·International Journal of Food Microbiology·B Rodríguez-PorrataR Cordero Otero
Jan 3, 2009·Biochimica Et Biophysica Acta·Fumiyoshi Abe, Toshiki Hiraki
Sep 1, 2009·Biochimica Et Biophysica Acta·Cally M ScherberAlptekin Aksan
Feb 2, 2010·Biochimica Et Biophysica Acta·Sebastien DupontPatrick Gervais
Jul 14, 2010·International Journal of Food Microbiology·Sara LandolfoIlaria Mannazzu
Nov 18, 2010·Biochimica Et Biophysica Acta·Sebastien DupontPatrick Gervais

❮ Previous
Next ❯

Citations

Aug 31, 2014·Applied Microbiology and Biotechnology·Sebastien DupontLaurent Beney
Jun 12, 2014·Journal of Pharmaceutical Sciences·Robert H WaltersSatoshi Ohtake
Sep 2, 2015·Journal of Biotechnology·Cyril IaconelliLaurent Beney
Dec 10, 2013·Applied Microbiology and Biotechnology·Marcia Regina da Silva PedriniPatrick Gervais
Jun 8, 2021·Comprehensive Reviews in Food Science and Food Safety·Leticia Casas-GodoyAnne Gschaedler-Mathis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.