PMID: 6411927Jul 1, 1983Paper

Air-drying of human leucocytes for scanning electron microscopy using the GTGO procedure

Journal of Microscopy
H GamlielA Polliack

Abstract

The utilization of tannic acid and guanidine hydrochloride as mordants for better osmium binding has been shown to serve as an excellent alternative to metal coating of organ tissue specimens for scanning electron microscopy (SEM). The present report describes the GTGO procedure, a modification of the TAO technique introduced by Murakami et al. (1977, 1978), which we have found successful for the preparation of air dried peripheral blood leucocytes for SEM studies. Air dried, GTGO-treated leucocytes show excellent preservation of surface features with minimal cell shrinkage. When critical point dried, GTGO-treated cells are examined, they also show less shrinkage than cells prepared with standard glutaraldehyde fixation and critical point drying. The potential application of this air drying procedure (GTGO-AD) to other soft biological specimens is currently under investigation. This technique is recommended as a new and effective air drying procedure for the successful preparation of cells for SEM.

References

Nov 1, 1975·The Journal of Cell Biology·S K SandersR C Braylan
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr

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Citations

Feb 1, 1985·The Histochemical Journal·J W CattF L Harrison
Dec 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A BirkenfeldI Vlodavsky
Aug 1, 1991·Biomaterials·K Gaebel, I A Feuerstein
Jul 1, 1988·Journal of Ultrastructure and Molecular Structure Research·M KesselU Aebi
Jan 16, 2004·Experimental Gerontology·Naiel AzzamAhuva Dovrat
Jan 1, 1990·Food Additives and Contaminants·G M WoodM O Moss
Nov 2, 2011·Biotechnology for Biofuels·Cheng YuanFuli Li
Oct 1, 1989·Journal of Periodontology·P A Farber
Sep 1, 1992·Leukemia & Lymphoma·S H WuH Gamliel
Jun 1, 1987·Biopolymers·G Marx
Jul 1, 1987·Toxicology and Applied Pharmacology·A SamaraE Fibach
May 1, 1986·International Journal of Cell Cloning·H MaozA J Treves
Apr 1, 1985·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·J YahalomI Vlodavsky

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