Air-Liquid Interface Method To Study Epstein-Barr Virus Pathogenesis in Nasopharyngeal Epithelial Cells

MSphere
Elizabeth A CavesKathy H Y Shair

Abstract

Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that establishes a latent reservoir in peripheral B-lymphocytes with sporadic reactivation. EBV also infects epithelial cells, predominantly resulting in a lytic infection, which may contribute to EBV transmission from saliva. In the nasopharynx, EBV infection can lead to the clonal expansion of a latently infected cell and the development of nasopharyngeal carcinoma (NPC). The mechanisms governing EBV pathogenesis in nasopharyngeal epithelium are largely unknown. An advanced understanding would depend on a physiologically relevant culture model of polarized airway epithelium. The recent application of the organotypic raft culture in keratinocytes has demonstrated great promise for the use of polarized cultures in the study of EBV permissive replication. In this study, the adaptation of an air-liquid interface (ALI) culture method using transwell membranes was explored in an EBV-infected NPC cell line. In the EBV-infected NPC HK1 cell line, ALI culture resulted in the completion of EBV reactivation, with global induction of the lytic cascade, replication of EBV genomes, and production of infectious progeny virus. We propose that the ALI culture method can be widely adopt...Continue Reading

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Citations

Aug 29, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tuna ToptanYuan Chang
Mar 7, 2021·Viruses·Yi Tian PngChwee Ming Lim
Jul 22, 2021·Microscopy Research and Technique·Silvia GroissDagmar Brislinger
Nov 9, 2021·PLoS Pathogens·Nicholas Van SciverShannon C Kenney

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Datasets Mentioned

BETA
KC207813.1

Methods Mentioned

BETA
biopsy
transfection
RNA-seq
PCR
transmission electron microscopy
scraping

Software Mentioned

Capture
StepOne
TopHat2
Cufflinks
CuffDiff2
gplots

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