Airborne particulate matter impairs corneal epithelial cells migration via disturbing FAK/RhoA signaling pathway and cytoskeleton organization

Nanotoxicology
Yu-Hong CuiHong-Wei Pan

Abstract

Cornea is the outmost structure of the eye and exposed directly to the air pollution. However, little is known about the effect of PM2.5 on corneal epithelium, which is critical for maintenance of cornea homeostasis and visual function. We investigated the influence of PM2.5 exposure on corneal epithelial migration and the possible mechanisms involved in the process. We observed wound healing in mouse model of cornea abrasion, evaluated the migration and mobility of cultured corneal epithelial cells with wound scratch assay and Transwell migration assay, detected the phosphorylation and interaction of FAK/paxillin with immunofluorescence and immunoprecipitation, and determined the RhoA activity and actin reorganization, in response to PM2.5 exposure. Exposure to PM2.5 remarkably inhibited corneal epithelial cell migration both in mouse model of corneal abrasion and in cell culture model. We found the phosphorylation and interaction of FAK/paxillin, RhoA activity as well as actin reorganization were suppressed by PM2.5 exposure. Moreover, formation of ROS might play a role in the action of PM2.5. PM2.5 exposure could result in delay of corneal epithelium wound healing by inhibiting cell migration, thus more attention should be p...Continue Reading

References

Nov 7, 1998·Ophthalmic Epidemiology·A D Négrel, B Thylefors
Aug 5, 2000·Progress in Retinal and Eye Research·L GermainS L Guérin
Jul 11, 2001·Experimental Biology and Medicine·L LuW W Kao
Feb 27, 2003·Progress in Retinal and Eye Research·Katsuyoshi SuzukiTeruo Nishida
Sep 2, 2003·Environmental Health Perspectives·Stephen H GavettM Ian Gilmour
Jan 1, 2004·American Journal of Respiratory Cell and Molecular Biology·Sophie BlanchetArmelle Baeza-Squiban
Apr 27, 2004·Experimental Eye Research·Robert M LavkerTung-Tien Sun
Jun 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Enzo Di IorioMichele De Luca
Aug 5, 2005·Environmental Health Perspectives·Ralph J DelfinoShaista Malik
Aug 5, 2005·Environmental Health Perspectives·Susanne BeckerYuh-Chin T Huang
Mar 9, 2006·JAMA : the Journal of the American Medical Association·Francesca DominiciJonathan M Samet
Jun 3, 2006·Environmental Monitoring and Assessment·Xinhua WangJiamo Fu
Jun 7, 2006·Epidemiology·Ulrike GehringH-Erich Wichmann
Nov 24, 2007·Seminars in Cell & Developmental Biology·P S Zelenka, P Arpitha
Feb 22, 2008·Neurotoxicology·Sheba M J MohanKumarBellina Veronesi
Jul 22, 2008·Investigative Ophthalmology & Visual Science·Aránzazu MedieroJesús Pintor
Aug 8, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chongze Ma, Manuela Martins-Green
Sep 19, 2009·Particle and Fibre Toxicology·Jesus A Araujo, Andre E Nel
May 12, 2010·Circulation·Robert D BrookUNKNOWN American Heart Association Council on Epidemiology and Prevention, Council on the Kidney in Cardiovascular Disease, and Coun
Nov 20, 2010·Investigative Ophthalmology & Visual Science·Qing PanMarjorie F Lou
Apr 27, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhijie LiC Wayne Smith
Dec 30, 2011·Environmental Research·Fernando Korn MalerbiAlfésio Luís Ferreira Braga
Dec 30, 2011·Investigative Ophthalmology & Visual Science·Chia-Jen ChangHsien-Yang Tsai
Nov 20, 2012·Journal of the Air & Waste Management Association·Jun-Ji CaoYong-Ming Han
Dec 19, 2012·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Hiroshi FujishimaHirohisa Saito
May 30, 2013·Environmental Health : a Global Access Science Source·Gerard HoekJoel D Kaufman
Jul 16, 2016·Investigative Ophthalmology & Visual Science·Yu-Hong CuiHong-Wei Pan

❮ Previous
Next ❯

Citations

May 18, 2019·Investigative Ophthalmology & Visual Science·Yu-Jun TangDavid Pei-Cheng Lin
Jun 26, 2020·Frontiers in Chemistry·Sundas Bahar YaqoobMohammad Rashid
Apr 8, 2020·Canadian Respiratory Journal : Journal of the Canadian Thoracic Society·Juwhan ChoiKyung Hoon Min

❮ Previous
Next ❯

Related Concepts

Related Feeds

Barrett Esophagus

Barrett’s esophagus if a serious complication of gastroesophageal reflux disease during which the normal esophageal lining changes to tissue that resembles intestinal lining. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.