Airway submucosal glands from cystic fibrosis swine suffer from abnormal ion transport across the serous acini, collecting duct, and ciliated duct

American Journal of Physiology. Lung Cellular and Molecular Physiology
Xiaojie LuanJuan P Ianowski

Abstract

The human airway is protected by an efficient innate defense mechanism that requires healthy secretion of airway surface liquid (ASL) to clear pathogens from the lungs. Most of the ASL in the upper airway is secreted by submucosal glands. In cystic fibrosis (CF), the function of airway submucosal glands is abnormal, and these abnormalities are attributed to anomalies in ion transport across the epithelia lining the different sections of the glands that function coordinately to produce the ASL. However, the ion transport properties of most of the anatomical regions of the gland have never been measured, and there is controversy regarding which segments express CFTR. This makes it difficult to determine the glandular abnormalities that may contribute to CF lung disease. Using a noninvasive, extracellular self-referencing ion-selective electrode technique, we characterized ion transport properties in all four segments of submucosal glands from wild-type and CFTR-/- swine. In wild-type airways, the serous acini, mucus tubules, and collecting ducts secrete Cl- and Na+ into the lumen in response to carbachol and forskolin stimulation. The ciliated duct also transports Cl- and Na+ but in the opposite direction, i.e., reabsorption from...Continue Reading

References

Dec 31, 1979·The Journal of Membrane Biology·J M Diamond
May 1, 1990·The Journal of Cell Biology·W M Kühtreiber, L F Jaffe
Jul 1, 1989·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·S Tripathi, E L Boulpaep
Jun 24, 1985·Neuroscience Letters·D Ammann, P Anker
Jul 1, 1984·Physiological Reviews·P H Barry, J M Diamond
Aug 1, 1994·The American Journal of Physiology·W E FinkbeinerJ H Widdicombe
Oct 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J P AudieJ P Aubert
Dec 1, 1993·The American Review of Respiratory Disease·L JorisP M Quinton
Jul 1, 1997·American Journal of Rhinology·M R AustS J Gendler
Jul 3, 1998·American Journal of Respiratory Cell and Molecular Biology·P SharmaJ F Engelhardt
Oct 22, 1998·European Journal of Human Genetics : EJHG·Q Jiang, J F Engelhardt
Dec 21, 2000·American Journal of Physiology. Cell Physiology·P J Smith, J Trimarchi
Feb 24, 2001·The Journal of Prosthetic Dentistry·S P Humphrey, R T Williamson
May 26, 2001·Pflügers Archiv : European journal of physiology·P Somieski, W Nagel
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·S JayaramanA S Verkman
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·N S JooJ J Wine
Aug 3, 2001·The Journal of Physiology·J M Doughty, P D Langton
Oct 9, 2002·Progress in Histochemistry and Cytochemistry·Kenji Saga
Oct 9, 2002·The Journal of Biological Chemistry·Nam Soo JooJeffrey J Wine
May 15, 2004·The Journal of Experimental Biology·Mark R Rheault, Michael J O'Donnell
Sep 17, 2004·The Journal of Experimental Biology·Juan P IanowskiMichael J O'Donnell
Feb 18, 2005·Molecular Biology of the Cell·Silvia M KredaRichard C Boucher
Aug 23, 2005·Proceedings of the American Thoracic Society·Jeffrey J Wine, Nam Soo Joo
Sep 26, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jin V WuJeffrey J Wine
Jun 15, 2007·Physiology·Paul M Quinton
Apr 21, 2009·Progress in Retinal and Eye Research·Darlene A Dartt
Dec 8, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert J Lee, J Kevin Foskett
Apr 30, 2010·Science Translational Medicine·David A StoltzMichael J Welsh
Aug 27, 2010·The Journal of Clinical Investigation·Xingshen SunJohn F Engelhardt
Aug 27, 2010·The Journal of Clinical Investigation·Robert J Lee, J Kevin Foskett
Aug 27, 2010·The Journal of Clinical Investigation·Nam Soo JooJeffrey J Wine
Mar 18, 2011·Science Translational Medicine·Lynda S OstedgaardDavid A Stoltz
Jun 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Omar A ItaniMichael J Welsh

❮ Previous
Next ❯

Citations

Dec 17, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Erik Hviid Larsen
Dec 16, 2020·American Journal of Respiratory and Critical Care Medicine·Kenichi OkudaRichard C Boucher
Jun 26, 2021·American Journal of Respiratory and Critical Care Medicine·Drake C BouzekDavid A Stoltz

❮ Previous
Next ❯

Software Mentioned

SIET
AMS
ASET
SPSS Statistics
ImageJ
Automated Scanning Electrode Technique ASET

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Lung Cellular and Molecular Physiology
E A CowleyD H Eidelman
American Journal of Physiology. Lung Cellular and Molecular Physiology
Jin V WuJeffrey J Wine
Physiological Reviews
Jonathan H Widdicombe, Jeffrey J Wine
Proceedings of the National Academy of Sciences of the United States of America
Xiaojie LuanJuan P Ianowski
© 2021 Meta ULC. All rights reserved