Airway surface liquid osmolality measured using fluorophore-encapsulated liposomes

The Journal of General Physiology
S JayaramanA S Verkman

Abstract

The airway surface liquid (ASL) is the thin layer of fluid coating the luminal surface of airway epithelial cells at an air interface. Its composition and osmolality are thought to be important in normal airway physiology and in airway diseases such as asthma and cystic fibrosis. The determinants of ASL osmolality include epithelial cell solute and water transport properties, evaporative water loss, and the composition of secreted fluids. We developed a noninvasive approach to measure ASL osmolality using osmotically sensitive 400-nm-diam liposomes composed of phosphatidylcholine/cholesterol/polyethylene glycol-phosphatidylcholine (1:0.3:0.08 molar ratio). Calcein was encapsulated in the liposomes at self-quenching concentrations (30 mM) as a volume-sensitive marker, together with sulforhodamine 101 (2 mM) as a volume-insensitive reference. Liposome calcein/sulforhodamine 101 fluorescence ratios responded rapidly (< 0.2 s) and stably to changes in solution osmolality. ASL osmolality was determined from calcein/sulforhodamine 101 fluorescence ratios after addition of microliter quantities of liposome suspensions to the ASL. In bovine airway epithelial cells cultured on porous supports at an air-liquid interface, ASL thickness (b...Continue Reading

References

Mar 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I Erjefält, C G Persson
Apr 1, 1989·Scandinavian Journal of Clinical and Laboratory Investigation·H GilljamB Strandvik
Mar 10, 1995·The Journal of Biological Chemistry·O SeksekA S Verkman
Jul 1, 1994·American Journal of Respiratory and Critical Care Medicine·R C Boucher
Jan 1, 1994·American Journal of Respiratory and Critical Care Medicine·P M Quinton
Dec 1, 1993·The American Review of Respiratory Disease·L JorisP M Quinton
Jan 28, 1998·American Journal of Respiratory and Critical Care Medicine·J HullP Phelan
Mar 11, 1998·The European Respiratory Journal·J H WiddicombeC Y Lee
Jan 29, 1999·Physiological Reviews·J M Pilewski, R A Frizzell
Feb 2, 1999·The Journal of Clinical Investigation·J J Wine
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·S BaconnaisE Puchelle
May 17, 2000·The Journal of Clinical Investigation·H MatsuiR C Boucher
Feb 13, 2001·The Journal of Clinical Investigation·S JayaramanA S Verkman

❮ Previous
Next ❯

Citations

Nov 9, 2006·The Journal of Membrane Biology·Horst Fischer, Jonathan H Widdicombe
Mar 3, 2005·Lung·Amelia W NgThomas A Heming
Sep 20, 2005·Pflügers Archiv : European journal of physiology·Peter Steen PedersenOle Frederiksen
Oct 5, 2001·Trends in Genetics : TIG·D J Davidson, M Rolfe
Nov 8, 2002·Trends in Molecular Medicine·Jens PoschetVojo Deretic
Nov 15, 2002·Journal of Anatomy·J H Widdicombe
Apr 15, 2009·The Journal of Biological Chemistry·Wan NamkungA S Verkman
Jan 5, 2002·The Journal of General Physiology·Ray A CaldwellPierre M Barker
Oct 15, 2003·The Journal of General Physiology·Yuanlin SongA S Verkman
Feb 26, 2004·The Journal of General Physiology·Bradley S Launikonis, D George Stephenson
Jun 16, 2011·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Helene HolmgrenEvert Ljungström
Oct 10, 2003·Current Opinion in Pulmonary Medicine·Scott H Donaldson, Richard C Boucher
Oct 14, 2003·American Journal of Respiratory and Critical Care Medicine·Ronald L GibsonBonnie W Ramsey
Sep 27, 2003·American Journal of Respiratory and Critical Care Medicine·Richard M EffrosReza Shaker
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Venkataramana K SidhayeLandon S King
Apr 11, 2007·Paediatric Respiratory Reviews·Peter T P Bye, Mark R Elkins
Jun 15, 2004·Biochemical and Biophysical Research Communications·Polani B Ramesh BabuThomas H Shaffer
Dec 21, 2005·Journal of Biotechnology·Madhavan NallaniUlrich Schwaneberg
Aug 21, 2014·Biophysical Journal·Liyan PingHoward C Berg
Feb 9, 2005·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·J M CourtneyJ S Elborn
Aug 18, 2017·Physiology·Paul M Quinton
Aug 12, 2010·Journal of Speech, Language, and Hearing Research : JSLHR·Kristine TannerJulie Wright-Costa
Jan 1, 2004·Journal of Speech, Language, and Hearing Research : JSLHR·Mahalakshmi Sivasankar, Kimberly V Fisher
Aug 7, 2007·Journal of Speech, Language, and Hearing Research : JSLHR·Mahalakshmi Sivasankar, Kimberly V Fisher
Apr 17, 2008·The Journal of Pharmacology and Experimental Therapeutics·Yi JingJeffrey S Fedan
Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·Karl Kunzelmann, Marcus Mall
Dec 12, 2002·American Journal of Physiology. Cell Physiology·A S VerkmanJay R Thiagarajah
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A S Verkman

❮ Previous
Next ❯

Methods Mentioned

BETA
size exclusion chromatography
Fluorescence
Transgenic
light scattering
confocal microscopy

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.