Ajuba: a new microtubule-associated protein that interacts with BUBR1 and Aurora B at kinetochores in metaphase

Biology of the Cell
Alexia FerrandDaniel Birnbaum

Abstract

The role of the LIM-domain-containing protein Ajuba was initially described in cell adhesion and migration processes and recently in mitosis as an activator of the Aurora A kinase. In the present study, we show that Ajuba localizes to centrosomes and kinetochores during mitosis. This localization is microtubule-dependent and Ajuba binds microtubules in vitro. A microtubule regrowth assay showed that Ajuba follows nascent microtubules from centrosomes to kinetochores. Owing to its contribution to mitotic commitment and its microtubule-dependent localization, Ajuba could also play a role during the metaphase-anaphase transition. We show that Ajuba interacts with Aurora B and BUBR1 [BUB (budding uninhibited by benomyl)-related 1], two major components of the mitotic checkpoint. Inhibition of BUBR1 by siRNA (small interfering RNA) disrupts chromosome alignment at the metaphase plate and modifies Ajuba localization due to premature mitotic exit. Ajuba is a microtubule-associated protein that collaborates with Aurora B and BUBR1 at the metaphase-anaphase transition and this may be important to ensure proper chromosome segregation.

References

Jan 1, 1987·Cell Motility and the Cytoskeleton·M BornensE Karsenti
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C BoscR L Margolis
Jun 7, 2000·The Journal of Biological Chemistry·L HerrerosP Sánchez-Mateos
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A SkoufiasR L Margolis
Apr 3, 2001·Nature Cell Biology·K B KaplanI S Näthke
Apr 3, 2002·Protoplasma·T KhuranaA A Noegel
May 30, 2002·The Journal of Cell Biology·Véronique ChevrierDidier Job
Nov 6, 2002·The Journal of Biological Chemistry·Helene MarieVania M M Braga
Apr 30, 2003·The Journal of Cell Biology·Claire DitchfieldStephen S Taylor
Jun 5, 2003·Journal of Cell Science·Ana CarvalhoSally P Wheatley
Jun 14, 2003·The EMBO Journal·Susanne M A LensRené H Medema
Sep 25, 2003·Cell Cycle·Susanne M A Lens, René H Medema
Nov 7, 2003·Oncogene·Nathalie ConteDaniel Birnbaum
Feb 10, 2004·Nature Cell Biology·Michael A LampsonTarun M Kapoor
Mar 6, 2004·Cancer Metastasis Reviews·William M Grady
Mar 17, 2004·Oncogene·Rajnish Bharadwaj, Hongtao Yu
Apr 6, 2004·Oncogene·Bénédicte DelavalDaniel Birnbaum
Sep 8, 2004·Chromosoma·Paola Vagnarelli, William C Earnshaw
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Julie L Kadrmas, Mary C Beckerle
Feb 25, 2005·The Journal of Cell Biology·Stephen J PrattGregory D Longmore
Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·S HonoreD Braguer
Jun 14, 2006·The Journal of Cell Biology·Gerben VaderSusanne M A Lens
Sep 7, 2007·Cell·Michael J Emanuele, P Todd Stukenberg

❮ Previous
Next ❯

Citations

Jul 8, 2015·International Journal of Molecular Sciences·Chou-Kit ChouChien-Chih Chiu
May 12, 2010·Biochemical and Biophysical Research Communications·Yoko NagaiHiroshi Nishina
Oct 20, 2009·Current Opinion in Cell Biology·Mar CarmenaWilliam C Earnshaw
May 27, 2016·The Journal of Biological Chemistry·Xingcheng ChenJixin Dong
Dec 30, 2020·Experimental Dermatology·Krista Schleicher, Daniel Schramek
Mar 9, 2010·Cell Division·Richa SinghYogeshwer Shukla

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.