AKAP-Lbc nucleates a protein kinase D activation scaffold

Molecular Cell
Graeme K CarnegieJohn D Scott

Abstract

The transmission of cellular signals often proceeds through multiprotein complexes where enzymes are positioned in proximity to their upstream activators and downstream substrates. In this report we demonstrate that the A-kinase anchoring protein AKAP-Lbc assembles an activation complex for the lipid-dependent enzyme protein kinase D (PKD). Using a combination of biochemical, enzymatic, and immunofluorescence techniques, we show that the anchoring protein contributes to PKD activation in two ways: it recruits an upstream kinase PKCeta and coordinates PKA phosphorylation events that release activated protein kinase D. Thus, AKAP-Lbc synchronizes PKA and PKC activities in a manner that leads to the activation of a third kinase. This configuration illustrates the utility of kinase anchoring as a mechanism to constrain the action of broad-spectrum enzymes.

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Citations

Apr 23, 2013·Pflügers Archiv : European journal of physiology·Kavisha AroraAnjaparavanda P Naren
Nov 19, 2013·Trends in Pharmacological Sciences·Jessica L Esseltine, John D Scott
Dec 31, 2005·Nature Cell Biology·Lei Chen, Robert S Kass
Nov 26, 2010·Nature Cell Biology·F Donelson SmithJohn D Scott
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Wei Wong, John D Scott
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Carine RossePeter J Parker
May 25, 2010·Nature Structural & Molecular Biology·Catherine T Pawson, John D Scott
Nov 3, 2010·The Journal of Biological Chemistry·Mariah H GoodallTerry B Rogers
Dec 16, 2010·The Journal of Biological Chemistry·Jianfen GuoSusan F Steinberg
Apr 5, 2011·The Journal of Biological Chemistry·Jeremy S LogueJohn D Scott
Dec 26, 2006·The Journal of Biological Chemistry·Maya T KunkelAlexandra C Newton
Sep 11, 2007·The Journal of Biological Chemistry·April S GoehringJohn D Scott
Dec 25, 2008·The Journal of Biological Chemistry·Adriana A Paulucci-HolthauzenKathleen L O'Connor
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Nov 15, 2011·Journal of Cardiovascular Pharmacology·Graeme K Carnegie, Brian T Burmeister
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Apr 7, 2010·Molecular Interventions·Emily J WelchJohn D Scott
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Nov 15, 2012·Molecular and Cellular Biology·F Donelson Smith, John D Scott
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Jun 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Aline Appert-CollinDario Diviani
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Mar 26, 2009·IUBMB Life·Graeme K CarnegieJohn D Scott
Oct 5, 2010·The FEBS Journal·Jeremy S Logue, John D Scott

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