Akt mediates insulin induction of glucose uptake and up-regulation of GLUT4 gene expression in brown adipocytes

FEBS Letters
R HernandezM Lorenzo

Abstract

Insulin acutely stimulated glucose uptake in rat primary brown adipocytes in a PI3-kinase-dependent but p70S6-kinase-independent manner. Since Akt represents an intermediate step between these kinases, this study investigated the contribution of Akt to insulin-induced glucose uptake by the use of a chemical compound, ML-9, as well as by transfection with a dominant-negative form of Akt (DeltaAkt). Pretreatment with ML-9 for 10 min completely inhibited insulin stimulation of (1) Akt kinase activity, (2) Akt phosphorylation on the regulatory residue Ser473 but not on Thr308, and (3) mobility shift in Akt1 and Akt2. However, ML-9 did not affect insulin-stimulated PI3-kinase nor PKCzeta activities. In consequence, ML-9 precluded insulin stimulation of glucose uptake and GLUT4 translocation to plasma membrane (determined by Western blot), without any effect on the basal glucose uptake. Moreover, DeltaAkt impaired insulin stimulation of glucose uptake and GFP-tagged GLUT4 translocation to plasma membrane in transiently transfected immortalised brown adipocytes and HeLa cells, respectively. Furthermore, ML-9 treatment for 6 h down-regulated insulin-induced GLUT4 mRNA accumulation, without affecting GLUT1 expression, in a similar fashi...Continue Reading

References

Nov 24, 1995·The Journal of Biological Chemistry·T HarutaJ M Olefsky
Mar 17, 1995·The Journal of Biological Chemistry·M NukagaT Sawai
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·K HaraT R Jackson
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J R Flores-RiverosM D Lane
Jul 10, 1999·The Journal of Biological Chemistry·A BarthelR A Roth
Mar 18, 2000·The Journal of Biological Chemistry·A Toker, A C Newton
May 2, 2000·The Journal of Biological Chemistry·K M SchubertV Duronio
Jul 21, 2000·The Journal of Clinical Investigation·J E Pessin, A R Saltiel
Oct 24, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·P NavarroM Lorenzo

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Citations

Mar 6, 2003·Developmental Biology·Melinda LarsenKenneth M Yamada
Jan 9, 2008·Seminars in Reproductive Medicine·Pinar H Kodaman, Antoni J Duleba
Jan 19, 2005·Acta Physiologica Scandinavica·A M ValverdeM Lorenzo
Jun 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Shubha VarmaWalter N Durán
Sep 15, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Parama DeySurinder K Batra
Jul 30, 2009·Journal of Lipid Research·Raju V S Rajala
Nov 8, 2005·Biological & Pharmaceutical Bulletin·Satoshi MoritoKen-ichi Miyamoto
Jul 17, 2008·Archives of Physiology and Biochemistry·Iria Nieto-VazquezMargarita Lorenzo
Mar 12, 2004·Biochemical and Biophysical Research Communications·Anne K JonassenMichael N Sack
Jun 21, 2015·Genomics, Proteomics & Bioinformatics·Shannon N TessierKenneth B Storey
Oct 13, 2004·Biochemical Pharmacology·Tetsuo AkibaKen-ichi Miyamoto
Aug 9, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christopher M SchonhoffM Sawkat Anwer
Jun 7, 2006·Archives of Physiology and Biochemistry·Sonia Fernández-VeledoMargarita Lorenzo
Dec 22, 2014·Lipids in Health and Disease·Andressa Bolsoni-LopesMaria Isabel C Alonso-Vale
Oct 22, 2016·Biochimica Et Biophysica Acta·Ester García-CasarrubiosÁngela M Valverde
Nov 24, 2016·Scientific Reports·Sébastien M LabbéMathieu Laplante
May 26, 2004·Endocrinology and Metabolism Clinics of North America·Gerd AssmannHelmut Schulte
Aug 13, 2004·Brain Research. Molecular Brain Research·Paul C Chin, Santosh R D'Mello
Oct 17, 2017·Journal of Endocrinological Investigation·W QuZ Jiang
Mar 5, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Sawkat Anwer
Dec 21, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Brian K BeseniMatlou P Mokgotho
May 30, 2002·The Journal of Biological Chemistry·Cynthia R L WebsterM Sawkat Anwer
Feb 5, 2005·Journal of Neuroscience Research·Kyle Johnson, Santosh R D'Mello
Feb 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xue-Lin CuiRonaldo P Ferraris
Mar 20, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L BouraouiI Navarro
Jan 26, 2002·The Journal of Biological Chemistry·Kei SakamotoLaurie J Goodyear
Mar 22, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Jing LiTerry L Vanden Hoek
Oct 21, 2004·American Journal of Physiology. Endocrinology and Metabolism·Shaikh Mizanoor RahmanJames M Ntambi
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Mar 21, 2008·The EMBO Journal·Sarah K KnutsonScott W Hiebert

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