Akt/eNOS and MAPK signaling pathways mediated the phenotypic switching of thoracic aorta vascular smooth muscle cells in aging/hypertensive rats

Physiological Research
Lin ZhangLijun Shi

Abstract

Considerable evidence demonstrates that phenotypic switching of vascular smooth muscle cells (VSMCs) is influenced by aging and hypertension. During phenotypic switching, VSMCs undergo a switch to a proliferative and migratory phenotype, with this switch being a common pathology in cardiovascular diseases. The aim of this study was to explore the joint influence of age and hypertension on thoracic aortic smooth muscle phenotypic switching and the balance of Akt and mitogen-activated protein kinase (MAPK) signaling during this switch. Different ages of spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) were used to establish hypertension and aging models. The phenotypic state was determined by detecting the marker proteins alpha-SM-actin, calponin, and osteopontin (OPN) via immunohistochemical staining and Western blot. Signaling proteins associated with the Akt and MAPK pathways were detected in rat thoracic aorta using Western blot. Both aging and hypertension caused a decrease in contractile (differentiated) phenotype markers (alpha-SM-actin and calponin), while the synthetic (proliferative or de-differentiated) phenotype maker was elevated (OPN). When combining hypertension and aging, this effect was enhanced,...Continue Reading

References

Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Elena A GoncharovaVera P Krymskaya
Jan 15, 2003·Progress in Cardiovascular Diseases·T K Jeffery, N W Morrell
Jul 23, 2004·Physiological Reviews·Gary K OwensBrian R Wamhoff
Mar 18, 2005·Journal of Cardiovascular Pharmacology·Shinji KawaharaMasunori Matsuzaki
Apr 7, 2006·Handbook of Experimental Pharmacology·M L Bochaton-Piallat, G Gabbiani
May 1, 2008·Circulation. Heart Failure·Mark RosenbergNorbert Frey
Jul 17, 2010·Pflügers Archiv : European journal of physiology·Matthias Barton
Apr 19, 2011·Clinical and Translational Science·Rachel L ElvebakWayne T Nicholson
Jul 30, 2011·Journal of Hypertension·Vasilios KotsisPeter Nilsson
Oct 29, 2011·Cell Biology International·Hong GaoKenneth S Ramos
Jan 24, 2012·International Journal of Cardiology·Mark RosenbergNorbert Frey
Apr 15, 2015·Journal of Molecular and Cellular Cardiology·Edward G Lakatta
Apr 28, 2016·The Canadian Journal of Cardiology·Adam HarveyRhian M Touyz
May 29, 2016·Physiological Reports·George LindesayChristine Vayssettes-Courchay
Sep 20, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qiang LiLi-Xia Liu

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Citations

Jan 31, 2020·Oxidative Medicine and Cellular Longevity·Qidong CaoChunli Song
Feb 28, 2020·Clinical Science·Macarena OrejudoMarta Ruiz-Ortega
Nov 17, 2020·Journal of the American Heart Association·Laura E BruijnJan H N Lindeman
Jul 22, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Chang-Meng WuYan-Wei Hu
Mar 30, 2021·Nefrología : publicación oficial de la Sociedad Española Nefrologia·Raúl R Rodrigues-DiezMarta Ruiz-Ortega
Jul 18, 2021·Nefrología : publicación oficial de la Sociedad Española Nefrologia·Raúl R Rodrigues-DiezMarta Ruiz-Ortega

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