AKT/mTOR as Novel Targets of Polyphenol Piceatannol Possibly Contributing to Inhibition of Proliferation of Cultured Prostate Cancer Cells.

ISRN Urology
Tze-Chen HsiehJoseph M Wu

Abstract

The polyphenol piceatannol has shown inhibition against tyrosine and serine/threonine kinases. Whether piceatannol also exerts activity on the mammalian target of rapamycin (mTOR), a kinase involved in growth control of eukaryotic cells, is not known. In this study, we tested the effects of piceatannol on proliferation of androgen-dependent (AD) LNCaP and androgen-independent (AI) DU145 and PC-3 prostate cancer (CaP) cells. Suppression of AD and AI CaP cell growth by piceatannol was accompanied by cell cycle blockade in G(1)/S and S phases for LNCaP and PC-3 and induction of apoptosis in DU145 cells. Induction of apoptosis by piceatannol in DU145 cells was evident by reduced expression of poly(ADP-ribose) polymerase (PARP), cleavage of caspase 3 and apoptosis inducing factor AIF, and an increase in cytochrome c. The apoptotic changes occurred in concordance with DNA damage, supported by increased phosphorylated histone H2AX. Immunoblot analyses showed that exposure of different-stage CaP cells to piceatannol also resulted in cell-type-specific downregulation of mTOR and its upstream and downstream effector proteins, AKT and eIF-4E-BP1. We propose that the observed AKT and mTOR changes are new targets of piceatannol possibly con...Continue Reading

References

Nov 30, 1989·Biochemical and Biophysical Research Communications·R L Geahlen, J L McLaughlin
Jun 23, 1997·FEBS Letters·N Pullen, G Thomas
Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Sep 2, 1998·International Journal of Cancer. Journal International Du Cancer·A M DiPietrantonioJ M Wu
Jul 30, 1999·Biochemical and Biophysical Research Communications·J Zheng, V D Ramirez
Dec 2, 1999·Experimental Cell Research·A Dufner, G Thomas
Apr 20, 2001·Seminars in Hematology·Z DarzynkiewiczP Smolewski
Aug 13, 2002·Nature Cell Biology·Ken InokiKun-Liang Guan
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazuhiro AshikawaBharat B Aggarwal
Feb 20, 2003·Journal of Agricultural and Food Chemistry·Emma CantosFrancisco A Tomás-Barberán
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasunari Takada, Bharat B Aggarwal
Jul 22, 2004·Journal of Agricultural and Food Chemistry·Agnes M RimandoJames R Ballington
Aug 17, 2004·European Journal of Nutrition·Mar LarrosaJuan Carlos Espín
May 12, 2005·Journal of Agricultural and Food Chemistry·Kuo-Lung KuChing-Yi Lien
Jul 14, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Levy KopelovichJames A Crowell
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jonathan R GledhillJohn E Walker
Nov 6, 2007·Biological & Pharmaceutical Bulletin·Takako Yokozawa, You Jung Kim
Aug 22, 2008·American Journal of Physiology. Cell Physiology·Henry E SpeichLisa K Jennings
Aug 30, 2008·The Journal of Clinical Investigation·Carolyn Waugh KinkadeCory Abate-Shen
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yong FuAmy S Lee
Apr 14, 2010·Current Cancer Drug Targets·L CiuffredaM Milella
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Luc FuricNahum Sonenberg

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Citations

Apr 16, 2013·BioMed Research International·Shailendra Kapoor
Jan 14, 2016·Journal of Agricultural and Food Chemistry·Mohamed Ali SeyedKavitha Vijayaraghavan
Aug 4, 2015·Neurochemistry International·Wei ZhaoGiulio Maria Pasinetti
Jan 29, 2014·Hematological Oncology·Frederic Carsten SchmeelIngo G H Schmidt-Wolf
Aug 25, 2016·Nutrients·Yue ZhouHua-Bin Li
Jul 5, 2018·Biology Open·Tal FrolingerGiulio Maria Pasinetti
Sep 22, 2019·International Journal of Molecular Sciences·Itika AroraTrygve O Tollefsbol
Apr 28, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tal FrolingerGiulio M Pasinetti
Apr 6, 2019·Current Stem Cell Research & Therapy·Yang CaoLingbo Kong
Mar 22, 2019·International Journal of Molecular Sciences·Adele ChimentoVincenzo Pezzi
Aug 3, 2021·Journal of Food Biochemistry·Andressa Mara BaseggioMário Roberto Maróstica Júnior

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Methods Mentioned

BETA
flow cytometry
protein assay

Software Mentioned

MultiCycle

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