Abstract
Insulinomas are pancreatic neuroendocrine tumors (pNET), usually benign. Akt/p27kip1 is an intracellular pathway overexpressed in many pNET. There are no data regarding its expression in human insulinomas. We aimed to investigate the expression of Akt and p27kip1 in 24 human insulinomas and to compare them to their expression in normal surrounding islets. Staining was performed on embedded paraffin tissue using polyclonal antibodies against total Akt, p-Akt, p27kip1, and pp27kip1. p-Akt was the predominant form in insulinomas; they presented lower Akt and p-Akt expression than normal islets in 83.3% and 87.5% of tumors, respectively. p27kip1 and pp27kip1 were mainly cytoplasmic in both insulinomas and normal tissue. Cytoplasmic pp27kip1 staining was higher in insulinomas and surprisingly nearly half of the insulinomas also presented nuclear p27kip1 (p = 0.029). No differences were observed in the subcellular localization of p27kip1 and activation of Akt between benign and malignant insulinomas. The low expression of Akt seen in insulinomas might explain the usual benign behavior of this type of pNET. Cytoplasmic p27kip1 in both insulinomas and normal islet cells could reflect the low rate of replication of beta cells, while nuc...Continue Reading
References
Apr 28, 1999·Endocrinology·J PetrikD J Hill
Sep 24, 2002·Nature Medicine·Incheol ShinCarlos L Arteaga
Sep 24, 2002·Nature Medicine·Jiyong LiangJoyce M Slingerland
Sep 24, 2002·Nature Medicine·Giuseppe VigliettoMassimo Santoro
Jul 24, 2004·American Journal of Physiology. Endocrinology and Metabolism·Lorna M Dickson, Christopher J Rhodes
Jan 18, 2005·Cancer Cell·Gustavo BaldassarreAlfonso Colombatti
Jun 14, 2005·Trends in Cell Biology·Camelia Iancu-Rubin, George F Atweh
Mar 11, 2006·Familial Cancer·Sandra FontanièreChang Xian Zhang
Nov 10, 2006·Cell Division·Laurence BockstaelePierre P Roger
Jul 20, 2007·Genes & Development·Peter SicinskiCarla Kim
Oct 31, 2007·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·H L O'Grady, K C Conlon
Apr 17, 2008·Cell Cycle·Stacy W Blain
Jul 2, 2008·Expert Reviews in Molecular Medicine·Abde M Abukhdeir, Ben Ho Park
Sep 5, 2008·Annual Review of Pathology·Nader Chalhoub, Suzanne J Baker
Jun 9, 2009·Endocrine-related Cancer·Emelyne DejeuxJörg Tost
Oct 16, 2009·Cell Cycle·Michelle D LarreaJoyce M Slingerland
Nov 6, 2009·Experimental & Molecular Medicine·Jinhwa Lee, Sung Soo Kim
Nov 18, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Edoardo MissiagliaAldo Scarpa
Mar 16, 2013·Diabetes·Nathalie M Fiaschi-TaeschAndrew F Stewart
Mar 1, 2014·Theranostics·Franziska Briest, Patricia Grabowski
Dec 9, 2014·Cell Cycle·Stefania BertonGustavo Baldassarre
Jan 31, 2016·Cell Cycle·Suzan Ruijtenberg, Sander van den Heuvel