Alanine, a Novel Growth Substrate for the Acetogenic Bacterium Acetobacterium woodii.

Applied and Environmental Microbiology
Judith Dönig, Volker Müller

Abstract

Acetogenic bacteria are an ecophysiologically important group of strictly anaerobic bacteria that grow lithotrophically on H2 plus CO2 or on CO or heterotrophically on different substrates such as sugars, alcohols, aldehydes, or acids. Amino acids are rarely used. Here, we describe that the model acetogen Acetobacterium woodii can use alanine as the sole carbon and energy source, which is in contrast to the description of the type strain. The alanine degradation genes have been identified and characterized. A key to alanine degradation is an alanine dehydrogenase which has been characterized biochemically. The resulting pyruvate is further degraded to acetate by the known pathways involving the Wood-Ljungdahl pathway. Our studies culminate in a metabolic and bioenergetic scheme for alanine-dependent acetogenesis in A. woodii IMPORTANCE Peptides and amino acids are widespread in nature, but there are only a few reports that demonstrated use of amino acids as carbon and energy sources by acetogenic bacteria, a central and important group in the anaerobic food web. Our finding that A. woodii can perform alanine oxidation coupled to reduction of carbon dioxide not only increases the number of substrates that can be used by this mod...Continue Reading

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Jan 21, 2016·Journal of Bacteriology·Dragan TrifunovićVolker Müller
Jul 14, 2018·Environmental Microbiology·Florian KrempVolker Müller

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Citations

Nov 13, 2019·Environmental Microbiology Reports·Dragan TrifunovićVolker Müller
Jan 27, 2021·Scientific Reports·Luise GöbbelsMarie Charlotte Schoelmerich
Apr 6, 2021·Environmental Microbiology·Dragan TrifunovićVolker Müller
May 15, 2021·Environmental Microbiology·Jimyung MoonVolker Müller
Aug 28, 2021·Environmental Microbiology·Jimyung Moon, Volker Müller

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