Jul 1, 1982

Alanine aminotransferase in bovine brain: purification and properties

Journal of Neurochemistry
M RuscákH Hager

Abstract

Mitochondrial and cytosolic alanine aminotransferases (EC 2.6.1.2) were partially purified (140- and 180-fold), respectively) from bovine brain cortex by means of (NH4)2SO4 precipitation, gel filtration on Sephadex G-150, and in-exchange chromatography on DEAE A-50 and characterized. The enzymes exhibited identical molecular weights (110,000 +/- 10,000) and pH optima (7.8), but were eluted from CM Sephadex C-50 at different ionic strengths. Isoelectric focusing of the enzymes indicated a pI value of 5.2 for the cytosolic enzyme and 7.2 for the mitochondrial enzyme. The Km values of the mitochondrial enzyme were 5.1 mM, 6.6 mM, 0.7 mM, and 0.4 mM and of the cytosolic isozyme were 30.3 mM, 4.3 mM, 0.7 mM, and 0.5 mM for alanine, glutamate, 2-oxoglutarate, and pyruvate, respectively. The results indicated that two forms of alanine aminotransferase exist in nerve tissue, which suggests that they may play different roles in the cellular metabolism of nerve tissue.

  • References22
  • Citations17

References

Mentioned in this Paper

Metabolic Process, Cellular
Sephadex G
Cortex Bone Disorders
Adrenal Cortex Diseases
Enzymes, antithrombotic
Alpha Ketoglutarate
Chromatography
Pyruvate Measurement
Brain
Bos taurus

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