Alanine and glutamine kinetics at rest and during exercise in humans

Medicine and Science in Sports and Exercise
B D WilliamsR R Wolfe

Abstract

The purpose of this study was to quantify both alanine and glutamine kinetics during exercise of moderate intensity to determine the sum total of alanine and glutamine flux. Tracer methods were used to quantify alanine and glutamine rates of appearance (Ra) in plasma at rest and during 180 min of approximately 45% VO2max treadmill exercise in six normal volunteers (25 +/- 2 yr, 68 +/- 2.5 kg, VO2max 43 +/- 2.4 mL.min-1.kg-1; means +/- SE). Bolus injections (N = 3) or primed-constant infusions (N = 3) of 2H5-glutamine and 3-13C-alanine were given at rest on 1 d and 10-15 min after the onset of exercise on a separate day less than 2 wk later. Plasma enrichment decay curves and plateau enrichments were used to estimate alanine and glutamine kinetics. Whereas alanine Ra increased significantly from rest to exercise (5.72 +/- 0.31 vs 13.5 +/- 1.9 mumol.min-1.kg-1, respectively; P < 0.01), glutamine Ra was not significantly altered by exercise (6.11 +/- 0.44 and 6.40 +/- 0.69 mumol.min-1.kg-1 at rest and during exercise, respectively). The total of alanine and glutamine flux increased from 17.93 +/- 0.88 to 25.98 +/- 3.04 (P < 0.05). Since most muscle amino-N is released as alanine and glutamine, these findings provide strong evidenc...Continue Reading

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Citations

May 13, 1998·Acta Physiologica Scandinavica·K D Tipton, R R Wolfe
Nov 18, 2006·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Sarah B WilkinsonStuart M Phillips
Oct 21, 2009·Current Opinion in Clinical Nutrition and Metabolic Care·Francesco Agostini, Gianni Biolo
Jul 26, 2002·The Japanese Journal of Physiology·C PetiboisG Déléris
May 9, 2014·PloS One·Maiko MoriJan Wernerman
Jul 20, 1999·Clinics in Sports Medicine·M H Williams

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