PMID: 6403992Mar 1, 1983Paper

Alanine-resistant mutants of Chinese hamster ovary cells, CHO-K1, producing increases in velocity of proline transport through the A, ASC, and P systems

Somatic Cell Genetics
J MoffettE Englesberg

Abstract

We have developed a method for the isolation of transport mutants with increases in velocity of transport through the A and ASC systems and through a newly discovered P system utilizing the amino acid antagonism between A system amino acids and proline in CHO-K1 pro- cells. Mutants alar2 and alar3, isolated in a single-step procedure, resistant to 25 mM alanine in MEM-10 plus 0.05 mM proline are pro-, stable, cross resistant to alpha-(methylamino)isobutyric acid (MeAIB) and show an approximately twofold increase in the initial velocity of proline uptake. Ethyl methane sulfonate (EMS) increases the frequency of pro- alar clones in the population by at least 50 times the spontaneous frequency. The increased velocity of proline transport by alar2 and alar3 can be attributable to the 1.5 to 3 times increase in velocity of transport of proline through systems A, ASC, and P. The Vmax for proline transport through the A system has increased two times for alar2 while the Km and Vmax for alar3 has increased by 1.4 and 2.3 times that of CHO-K1. There is a corresponding increase in Vmax of proline transport by alar2 through the P system. The P system is defined operationally as that portion of the Na+-dependent velocity that remains when ...Continue Reading

References

Dec 15, 1978·Biochimica Et Biophysica Acta·G G GuidottiG C Gazzola
Jan 1, 1977·Journal of Neurochemistry·E G ArcherM N Sharata

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Citations

Dec 1, 1989·Journal of Cellular Physiology·M J Campa, M S Kilberg
Jan 1, 1986·The Journal of Membrane Biology·E Englesberg, J Moffett
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J MoffettE Englesberg
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N X QianE Englesberg
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N X QianE Englesberg
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M JonesE Englesberg
Mar 1, 1991·Journal of Cellular Physiology·B M RotoliG C Gazzola
Jan 15, 1987·Biochemical and Biophysical Research Communications·M Feneant-ThibaultA Lemonnier
Dec 9, 1991·Biochimica Et Biophysica Acta·O BussolatiG C Gazzola
Jan 22, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Arkadiusz SurazyńskiJerzy Pałka

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