PMID: 9525683Apr 3, 1998Paper

Alanine substitutions of polar and nonpolar residues in the amino-terminal domain of CCR5 differently impair entry of macrophage- and dualtropic isolates of human immunodeficiency virus type 1

Journal of Virology
G E RabutT Dragic

Abstract

Multiple extracellular domains of the CC-chemokine receptor CCR5 are important for its function as a human immunodeficiency virus type 1 (HIV-1) coreceptor. We have recently demonstrated by alanine scanning mutagenesis that the negatively charged residues in the CCR5 amino-terminal domain are essential for gp120 binding and coreceptor function. We have now extended our analysis of this domain to include most polar and nonpolar amino acids. Replacement of alanine with all four tyrosine residues and with serine-17 and cysteine-20 decrease or abolish gp120 binding and CCR5 coreceptor activity. Tyrosine-15 is essential for viral entry irrespective of the test isolate. Substitutions at some of the other positions impair the entry of dualtropic HIV-1 isolates more than that of macrophagetropic ones.

References

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Citations

Jul 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·M MoulardJ M Sabatier
Aug 29, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·J C Martín, J C Bandrés
Feb 28, 2002·Journal of Virology·Daniah A D ThompsonTatjana Dragic
Sep 10, 2002·AIDS·Julio Martín-GarcíaFrancisco González-Scarano
Apr 23, 2004·Proteins·Shang-Te D Hsu, Alexandre M J J Bonvin
Dec 22, 2007·Molecular Pharmacology·Rama KondruMarianna Dioszegi
Jun 8, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Abdelaziz S A AbuelsaadAhmed S Abdel-Moneim
Apr 26, 2014·PloS One·Phanourios Tamamis, Christodoulos A Floudas
Apr 1, 2015·AIDS Research and Human Retroviruses·Giselle Calasans de Souza CostaLuiz Carlos Junior Alcantara
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·E G CormierT Dragic
Nov 19, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Tonie Cilliers, Lynn Morris
Feb 18, 2006·PLoS Computational Biology·Yang ZhangJeffrey Skolnick
May 6, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Asongna T FolefocColleen A Flanagan
Jan 9, 2010·AIDS Research and Human Retroviruses·John C TiltonRobert W Doms
Sep 3, 2009·AIDS·Pierre Corbeau, Jacques Reynes
Aug 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christoph SeibertThomas P Sakmar
Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·T DragicJ P Moore
May 22, 2009·Expert Opinion on Therapeutic Patents·Maja A Sommerfelt
Sep 10, 2011·AIDS Research and Human Retroviruses·Maria Espirito-SantoJ Miguel Azevedo-Pereira

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