Alantolactone induces apoptosis and suppresses migration in MCF‑7 human breast cancer cells via the p38 MAPK, NF‑κB and Nrf2 signaling pathways

International Journal of Molecular Medicine
Jianli LiuXiangyu Cao

Abstract

Human breast cancer is a malignant type of cancer with high prevalence. In the present study, the anticancer effects of alantolactone, a sesquiterpene lactone, on the human breast cancer cell line MCF‑7 were investigated in vitro. The MCF‑7 cell morphology changed from diamond to round subsequent to treatment with alantolactone, and the cell viability reduced significantly compared with that of the control cells. Alantolactone induced apoptosis of MCF‑7 cells by regulating the protein expression levels of B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein, p53, caspase‑3 and caspase‑12, which are associated with the apoptotic pathway, and suppressed colony formation and migration by regulating the protein expression of matrix metalloproteinase (MMP)‑2, MMP‑7 and MMP‑9. Cell signaling pathway analysis confirmed that alantolactone increased the phosphorylation of p38, and decreased the nuclear expression levels of p65 and nuclear factor erythroid 2‑related factor 2 (Nrf2), suggesting that the apoptosis‑promoting and migration‑suppressing effect of alantolactone may partially depend on regulating the p38 MAPK, NF‑κB and Nrf2 pathways. These results also suggested that alantolactone may become a potential therapeutic strategy fo...Continue Reading

References

Jul 23, 2002·Biological & Pharmaceutical Bulletin·Krzysztof BielawskiSławomir Wołczyński
Oct 24, 2002·Biological & Pharmaceutical Bulletin·Tenji KonishiTakao Konoshima
Mar 22, 2007·Journal of Agricultural and Food Chemistry·Hyungeun Yoon, Rui Hai Liu
Nov 6, 2009·Journal of Pharmaceutical and Biomedical Analysis·Yan HuoXiaobo Li
May 29, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ya-Fan LiaoYew-Min Tzeng
Jun 23, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jia-Chuan LeiGuo-Lin Zou
Jan 10, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Eun Jung ParkKi Churl Chang
Aug 8, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Azhar RasulXiaomeng Li
Oct 19, 2013·Experimental and Therapeutic Medicine·Weimin LuoJialong Guo
Jan 20, 2015·Journal of Biochemical and Molecular Toxicology·Peng ZhaoXingyi Zhang
May 29, 2015·International Journal of Molecular Sciences·Na ZhouHuanran Tan
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yali LiChengjian Zhao
Jan 26, 2017·Acta Pharmaceutica Sinica. B·Tiancong WuXixu Zhu
May 6, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Seema KumariRamaRao Malla
Apr 1, 2018·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Neal ShahPaul R Lockman
Apr 8, 2018·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Somchaiya SurichanVasilis P Androutsopoulos

❮ Previous
Next ❯

Citations

Jun 21, 2020·Antioxidants·Ekaterina ProshkinaAlexey Moskalev
Jul 15, 2020·Recent Patents on Anti-cancer Drug Discovery·Elaheh AminiAbolfazl Shakeri
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Karin Lunz, Iris Stappen

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
protein assay

Software Mentioned

ImageJ
SPSS

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.