PMID: 15348506Sep 7, 2004Paper

Albumin adsorption on cibacron blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers

Journal of Materials Science. Materials in Medicine
M Cristina L MartinsM A Barbosa

Abstract

Self-assembled monolayers can be tailored with specific ligands to a certain protein and at the same time prevent the non-specific adsorption of other proteins. Cibacron Blue F3G-A (CB-thiol) was successfully immobilized onto tetra(ethylene glycol)-terminated alkanethiol (CB-thiol). The affinity of human serum albumin (HSA) to immobilized Cibacron Blue F3G-A was studied using mixed thiolate self-assembled monolayers on gold with different n-alkyl chain lengths and functional terminal groups (CH(3)-; OH- and tetra(ethylene glycol)). Surfaces were characterized using X-ray photoelectron spectroscopy and water contact angle measurements. Albumin adsorption and exchangeability of the adsorbed albumin molecules with other albumin molecules in solution were evaluated using (125)I-radiolabeled HSA. Competitive adsorption between albumin and fibrinogen to the different self-assembled monolayers (SAMs) was also investigated. Results showed that the incorporation of CB-thiol on the monolayers does not increase the HSA adsorption and reversibility on the SAMs. However, although specific adsorption of HSA to the immobilized Cibacron Blue F3G-A was not demonstrated, the presence of CB-thiol decreases the affinity of fibrinogen to the OH-ter...Continue Reading

Citations

Mar 20, 2018·International Journal of Dentistry·Sidónio C FreitasAlejandro Pelaez-Vargas
Dec 7, 2005·Journal of Biomedical Materials Research. Part a·Judite N BarbosaArtur P Aguas
Mar 26, 2010·Journal of Biomedical Materials Research. Part a·Joana MacielMário A Barbosa
Jul 15, 2011·Journal of Materials Science. Materials in Medicine·Inês C GonçalvesBuddy D Ratner
Sep 8, 2010·Journal of Biomaterials Applications·Sanjukta Guha ThakurtaAnuradha Subramanian
Dec 2, 2010·Journal of Materials Science. Materials in Medicine·Sanjukta Guha Thakurta, Anuradha Subramanian
Aug 17, 2010·Journal of Colloid and Interface Science·Sagheer A OnaiziAnton P J Middelberg

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