Albumin-derived nanocarriers: substrates for enhanced cell adhesive ligand display and cell motility

Biomaterials
Ram I SharmaPrabhas V Moghe

Abstract

Cell-adhesive ligands organized on nanoscale synthetic biomaterials can potentially recapitulate the nanoscale organization of extracellular matrix and the consequent effects of cell dynamics. In this study, 100 nm albumin nanocarriers (ANC) were fabricated to serve as nanoscale organizational units for a well-defined ligand, the recombinant fragment from fibronectin comprised of the RGD-containing module 10 and the synergy-region-containing module 9. Conventional protein conjugation chemistry was employed to fabricate nanocarriers with increasing levels of displayed ligand. Presentation of ligand-functionalized ANCs adsorbed onto substrates was found to enhance keratinocyte attachment when compared to equivalent levels of adsorbed ligands, supported by ELISA data that the display of ligand on ANCs essentially increased the accessibility of the cell-binding domain and AFM data that the ligand was likely exposed due to ligand-ANC repulsion. The ligand presentation from ANCs converted the cellular morphology from a stationary phenotype to a motile phenotype, with the expression of filopodia-like microextensions, and a decrease in focal adhesions, indicating decreased cell adhesion strength. Consequently, cell motility was found t...Continue Reading

Citations

Oct 31, 2008·Journal of Pharmaceutical Sciences·Farnaz EsmaeiliFatemeh Atyabi
Dec 6, 2011·Colloids and Surfaces. B, Biointerfaces·Shaw-Ruey LyuChia-Wei Chuang
Jan 17, 2015·Colloids and Surfaces. B, Biointerfaces·Marilene EstanqueiroJosé Manuel Sousa Lobo

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