Albumin endocytosis in OK cells: dependence on actin and microtubules and regulation by protein kinases

The American Journal of Physiology
M GekleS Silbernagl

Abstract

We used proximal tubule-derived opossum kidney (OK) cells to determine the dependence of albumin endocytosis on regulation by protein kinases and on the cytoskeleton. Uptake was observed only across the apical but not the basolateral membrane and exceeded uptake in collecting duct-derived Madin-Darby canine kidney cells 14-fold. Inhibition of endocytosis via clathrin-coated vesicles but not via caveolae abolished uptake. Cytochalasin D reduced uptake to < 5% of control, and inhibition of microtubule polymerization by nocodazole reduced uptake to approximately 55% of control. Activation of protein kinase A (PKA) by adenosine 3',5'-cyclic monophosphate, forskolin, or parathyroid hormone (PTH) reduced uptake to approximately 65% of control. Protein kinase C (PKC) activation did affect uptake to a similar extent as PKA activation but with a certain delay. Stimulation of PKG by guanosine 3',5'-cyclic monophosphate did not affect albumin endocytosis. The inhibitor of tyrosine kinases (TRK), genistein, induced an increase of uptake to approximately 160% of control. Reexocytosis of albumin was enhanced by PKC activation but not by PKA activation. TRK inhibition reduced the rate of reexocytosis. We conclude that albumin endocytosis in O...Continue Reading

Citations

Jul 15, 2003·Molecular Biology of the Cell·Laura AschenbrennerTama Hasson
Jan 15, 2015·American Journal of Physiology. Renal Physiology·Aven LeePhilip Poronnik
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Daniel BiemesderferTama Hasson
Feb 16, 2005·Annual Review of Physiology·Michael Gekle
Apr 29, 2004·American Journal of Physiology. Renal Physiology·Michael GekleMartin Wiemann
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Michael GekleErik I Christensen
Jan 20, 2005·American Journal of Physiology. Renal Physiology·Deanne H HryciwPhilip Poronnik
Jul 19, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M CasartelliB Giordana
Jun 3, 2021·International Journal of Molecular Sciences·Jakub GburekKrzysztof Gołąb
May 27, 2021·The Journal of Physiology·Ora A Weisz

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