PMID: 9419017Jan 7, 1998Paper

Albumin enhances superoxide production in cultured microglia

Glia
Q S SiK Kataoka

Abstract

Microglial activation and disruption of blood-brain barrier (BBB) are known to occur and contribute to neuronal damages in cerebral ischemic conditions and in some neurodegenerative diseases. To investigate whether a serum factor leaked out from circulation enhances microglial activation, we examined the effect of normal rat serum on superoxide (O2-) production by cultured microglia. Microglia cultured from neonatal rat brains were studied on their O2- production induced by the addition of phorbol myristate acetate by a method of acetyl-cytochrome c reduction. The O2- production was significantly increased by the addition of 0.01% rat serum, and the maximal enhancement was observed at about 0.1% rat serum. After the serum was fractionated using a molecular sieve membrane, we observed the enhancing effect only in a greater molecular weight fraction (>50 kDa). Furthermore, three kinds of bovine serum albumin (BSA) with different purity, and human serum and plasma albumins, also enhanced O2- production to a similar extent to that by rat serum. However, other proteins tested showed no significant effect. The enhancement of O2- production by BSA was observed dose-dependently, and the effect of 50 microg/ml of purified BSA was equiva...Continue Reading

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Citations

Jun 30, 2000·Biochemical and Biophysical Research Communications·Y KataokaY Watanabe
Aug 22, 2009·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Tian-zhi ZhaoJiang-kai Lin
Dec 19, 1998·Neuroscience Research·K Kataoka, H Yanase
Jun 17, 2003·Biological & Pharmaceutical Bulletin·Masaki HirashimaKazuhiko Takahashi
Dec 8, 2009·Brain Research·Hantamalala Ralay Ranaivo, Mark S Wainwright
May 10, 2005·Journal of Neuroscience Research·Charles-Félix CalvoJacques Glowinski
Mar 12, 2011·Developmental Neurobiology·Maria-Carmen CarrascoJosé L Marín-Teva
Jan 8, 1999·Experimental Neurology·S SudoN Maeda
Jan 31, 2007·Stroke; a Journal of Cerebral Circulation·Kenneth R Wagner
Feb 13, 2021·Frontiers in Neurology·Diogo Vila VerdeLaura Librizzi

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