PMID: 6171876Jul 1, 1981Paper

Albumin-immunoglobulin complexes in human serum: classification and immunochemical analysis

Scandinavian Journal of Immunology
D EilatA Zlotnick

Abstract

A complex between serum albumin and immunoglobulins was observed on immunoelectrophoresis in six patients. Two patients with multiple myeloma had monoclonal IgA-albumin complexes; one of these complexes was formed by covalent bonds and the other by non-covalent bonds. Four patients displayed non-covalent IgG-albumin complexes: of these, one had multiple myeloma, two had been treated with nitrofurantoin for prolonged periods, and one had diabetes mellitus. The IgG-albumin complex of the last patient was subjected to a detailed immunochemical analysis. The albumin-specific antibodies were isolated by affinity chromatography and analysed, using a sensitive tritium labelling technique. The antibodies were polyclonal, complexed with serum albumin through their Fab portion, and showed a high specificity for the human albumin as compared with bovine and rabbit albumins. The serum albumin of two patients displayed an abnormal behaviour in reduced SDS-polyacrylamide gel electrophoresis (PAGE). The abnormal albumins had an apparent molecular weight of 52,000 and could react with rabbit anti-human serum albumin like the normal protein. No abnormal albumin could be detected in 20 other patients' sera, including nitrofurantoin-treated patie...Continue Reading

References

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Citations

Mar 1, 1993·International Journal of Dermatology·V N Sehgal, A K Chaudhry
Apr 24, 2003·Clinical Chemistry·Kiyotaka FujitaTsutomu Katsuyama
Jul 1, 1996·European Journal of Immunology·E K Kristoffersen, R Matre
Dec 1, 1983·European Journal of Immunology·H S ZhangA C Wang
Jan 12, 1983·Biochimica Et Biophysica Acta·J P SalierJ P Martin

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