Alcian yellow as a fluorescent dye

Acta histochemica
J C StockertR Armas-Portela

Abstract

Fluorescence characteristics of the cationic dye Alcian yellow are described. Under ultraviolet excitation, the chromatin and basophilic cytoplasm from cell smears show a blue-white emission, which depends on the presence of nucleic acids. Glycosaminoglycans-containing structures (mast cell granules, cartilage matrix) appear brightly fluorescent. The excitation at 320 less than or equal to lambda less than or equal to 340 nm is the most suitable, and the emission wavelength shows dependence on the dye concentration.

References

Jan 1, 1977·Stain Technology·R D Lillie
Oct 1, 1986·Journal of Microscopy·M L MoleroJ C Stockert
Jan 1, 1986·Histochemistry·P del CastilloJ C Stockert
Mar 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Kelényi

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Citations

Apr 1, 1995·Acta histochemica·M L MoleroJ C Stockert

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