Alcohol binding to liposomes by 2H NMR and radiolabel binding assays: does partitioning describe binding?

Biophysical Journal
A K DubeyN Janes

Abstract

Implicit within the concept of membrane-buffer partition coefficients of solutes is a nonspecific solvation mechanism of solute binding. However, (2)H NMR studies of the binding of (2)H(6)-ethanol and [1-(2)H(2)] n-hexanol to phosphatidylcholine vesicles have been interpreted as evidence for two distinct alcohol binding modes. One binding mode was reported to be at the membrane surface. The second mode was reported to be within the bilayer interior. An examination of the (2)H NMR binding studies, together with direct radiolabel binding assays, shows that other interpretations of the data are more plausible. The results are entirely consistent with partitioning (nonspecific binding) as the sole mode of alcohol binding to liposomes, in accord with our previous thermodynamic interpretation of alcohol action in phosphatidylcholine liposomes.

Citations

Dec 24, 1996·Biochemical Pharmacology·S ChannareddyN Janes
Apr 13, 1999·Trends in Neurosciences·J Cudeiro, C Rivadulla
Jun 6, 2003·Biochimica Et Biophysica Acta·Nuno C SantosMiguel A R B Castanho
Feb 17, 2000·Biochimica Et Biophysica Acta·S ChannareddyN Janes
Jul 26, 1996·The Journal of Biological Chemistry·S ChannareddyN Janes
Apr 17, 1997·The Journal of Physical Chemistry. B·M A Wilson, A Pohorille

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